U-Blot® Myosin Light Chain 2 Rabbit mAb
WR4228 Western blot analysis of lysates from B16F10 cells, using MelanA Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:This gene encodesThe regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggersThe phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | MYL2 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 18kD |
| MW(Observed) | 18kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:400-1:1000; WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0348R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | MYL2 |
| Protein Name | Myosin regulatory light chain 2 ventricular/cardiac muscle isoform |
| Other Name | MYL2; / Myosin regulatory light chain 2; / ventricular/cardiac muscle isoform; / MLC-2; / MLC-2v |
| Species | Human |
| Gene ID | 2315 |
| Uniprot | P10916, |
| Species.1 | Mouse |
| Gene ID.1 | 17906 |
| Uniprot.1 | P51667 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm |
| Tissue Expression | Expression is restricted to melanoma and melanocyte cell lines and retina. |
| Signaling_pathway | |
| Research Areas | |
| Function | Disease:Defects in MYL2 are the cause of cardiomyopathy familial hypertrophic type 10 (CMH10) [MIM:608758]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.,Disease:Defects in MYL2 are the cause of cardiomyopathy hypertrophic with mid-left ventricular chamber type 2 (MVC2) [MIM:608758]. MVC2 is a very rare variant of familial hypertrophic cardiomyopathy, characterized by mid-left ventricular chamber thickening.,miscellaneous:This chain binds calcium.,similarity:Contains 3 EF-hand domains.,subunit:Myosin is an hexamer of 2 heavy chains and 4 light chains., |
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