U-Blot® Nrf1 Rabbit mAb
WR4483 Hela whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Price range: $268.00 through $328.00
Product details
Background:This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014],
Specifications
| Size | 50μL/100μL |
| Target | NRF-1 |
| Reactivity | Human, Mouse, Rat, Bovine |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 54kD |
| MW(Observed) | 68kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0704R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | NRF1 |
| Protein Name | Nuclear respiratory factor 1 |
| Other Name | NRF1; / Nuclear respiratory factor 1; / NRF-1; / Alpha palindromic-binding protein; / Alpha-pal |
| Species | Human |
| Gene ID | 4899 |
| Uniprot | Q16656, |
| Species.1 | Mouse |
| Gene ID.1 | 18181 |
| Uniprot.1 | Q9WU00, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q62792 |
| Cellular Localization | Nucleus |
| Tissue Expression | Ubiquitously expressed with strongest expression in skeletal muscle. |
| Signaling_pathway | Human Diseases >> Neurodegenerative disease >> Huntington disease;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway |
| Research Areas | >>Apelin signaling pathway; / >>Huntington disease |
| Function | Function:Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.,PTM:Phosphorylation enhances DNA binding.,similarity:Belongs to the NRF1/Ewg family.,subunit:Homodimer. Binds DNA as a dimer. Interacts with PPRC1.,tissue specificity:Ubiquitously expressed with strongest expression in skeletal muscle., |
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