Name: U-Blot® Osteopontin Rabbit mAb
SKU: WR4255
Price: 268 USD
Availability: InStock

U-Blot® Osteopontin Rabbit mAb

Western blot analysis of lysates from A549 cells, using Osteopontin Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

WR4255 Western blot analysis of lysates from A549 cells, using Osteopontin Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4255-50

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Product details

Background:secreted phosphoprotein 1(SPP1) Homo sapiens The protein encoded by this gene is involved inThe attachment of osteoclasts toThe mineralized bone matrix.The encoded protein is secreted and binds hydroxyapatite with high affinity.The osteoclast vitronectin receptor is found inThe cell membrane and may be involved inThe binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],

Specifications

Size	50μL/100μL
Target	OPN
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	35kD
MW(Observed)	55,65kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0442R
Immunogen	AA range: 17-100
Specificity	Endogenous
Gene Name	SPP1
Protein Name	Osteopontin
Other Name	SPP1; / BNSP; / OPN; / PSEC0156; / Osteopontin; / Bone sialoprotein 1; / Nephropontin; / Secreted phosphoprotein 1; / SPP-1; / Urinary stone protein; / Uropontin
Species	Human
Gene ID	6696
Uniprot	P10451,
Species.1	Mouse
Gene ID.1	20750
Uniprot.1	P10923,
Species.2	Rat
Gene ID.2	25353
Uniprot.2	P08721
Cellular Localization	Secreted
Tissue Expression	Bone. Found in plasma.
Signaling_pathway	Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> ECM-receptor interaction
Research Areas	>>PI3K-Akt signaling pathway; / >>Apelin signaling pathway; / >>Focal adhesion; / >>ECM-receptor interaction; / >>Toll-like receptor signaling pathway; / >>GnRH secretion; / >>Human papillomavirus infection
Function	Disease:This protein plays a principal role in urinary stone formation as the stone matrix.,Function:Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.,Function:Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.,online information:Osteopontin entry,PTM:Extensively phosphorylated on clustered serine residues.,PTM:N- and O-glycosylated.,similarity:Belongs to the osteopontin family.,subunit:Ligand for integrin alpha-V/beta-3.,