p120 catenin [ABT082] mouse mAb
Ucallm_WM1490-50
Price range: $268.00 through $328.00
Product details
Background:
α- Catenin、 β- Catenin、 γ- catenin and p120 catenin are members of the connexin family and mediate intercellular adhesion together with E-cadherin. P120 catenin binds to the near membrane region of E-cadherin to form a complex, which is stable and closely linked. In lobular breast cancer, E-cadherin deficiency resulted in the accumulation of p120 catenin in the cytoplasm, while in ductal breast cancer, p120 catenin in the membrane decreased and there was no accumulation of p120 catenin in the cytoplasm. Therefore, it can be used to distinguish lobular carcinoma from ductal carcinoma. Studies have shown that p120 catenin in the cytoplasm of gastric and colon cancer is related to poor adhesion of cancer cells.
Specifications
| Size | 50μL/100μL |
| Target | p120 Catenin |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 108kD |
| MW(Observed) | 108kD |
| Host Species | Mouse |
| Isotype | IgG2b,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM082 |
| Immunogen | Synthesized peptide derived from human p120 catenin AA range: 600-700 |
| Specificity | The antibody can specifically recognize human p120 catenin protein. In western blotting of Hela, A431 and HEK293 cell lysates, the antibody can label a band with molecular weight around 100~110 kDa. |
| Gene Name | CTNND1 KIAA0384 |
| Protein Name | p120 catenin |
| Other Name | Cadherin associated Src substRate; / Cadherin-associated Src substRate; / CAS; / Catenin; / cadherin associated protein; / delta 1; / Catenin delta 1; / Catenin delta; / Catenin delta-1; / CTND1_HUMAN; / CTNND 1; / CTNND; / CTNND1; / delta 1 Catenin; / KIAA0384; / p120; / P120 CAS; / p120 catenin; / P120 CTN; / p120; / cas; / p120; / ctn; / P120CAS; / P120CTN |
| Species | Human |
| Gene ID | 1500 |
| Uniprot | O60716 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasmic, Membranous |
| Tissue Expression | Expressed in vascular endothelium. Melanocytes and melanoma cells primarily express the long isoform 1A, whereas keratinocytes express shorter isoforms, especially 3A. The shortest isoform 4A, is detected in normal keratinocytes and melanocytes, and generally lost from cells derived from squamous cell carcinomas or melanomas. The C-terminal alternatively spliced exon B is present in the p120ctn transcripts in the colon, intestine and prostate, but lost in several tumor tissues derived from these organs. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway |
| Research Areas | >>Rap1 signaling pathway; / >>Adherens junction; / >>Leukocyte transendothelial migration |
| Function | Alternative products:Experimental confirmation may be lacking for some isoforms,Disease:May contribute to cell malignancy. Complete loss of expression was observed in approximately 10% of invasive ductal breast carcinomas investigated.,Domain:A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.,Function:Binds to and inhibits the transcriptional repressor ZBTB33, which may lead to activation of target genes of the Wnt signaling pathway (By similarity). May associate with and regulate the cell adhesion properties of both C- and E-cadherins. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors. Promotes GLIS2 C-terminal cleavage.,induction:Induced in vascular endothelium by wounding. This effect is potentiated by prior laminar shear stress, which enhances wound closure.,PTM:Phosphorylated.,similarity:Belongs to the beta-catenin family.,similarity:Contains 10 ARM repeats.,subcellular location:Interaction with GLIS2 promotes nuclear translocation.,subunit:Belongs to a multiprotein cell-cell adhesion complex that also contains E-cadherin, alpha-catenin, beta-catenin, and gamma-catenin. Binds to the C-terminal fragment of PSEN1 and mutually competes for E-cadherin. Interacts with ZBTB33. Interacts with GLIS2.,tissue specificity:Expressed in vascular endothelium., |
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