Name: PIK3CA H1047R [ABT454] mouse mAb
SKU: WM1506
Price: 268 USD
Availability: InStock

Product details

Background:
The PIK3CA gene is located at 3Q26.3 and encodes a 124 kDa protein. The protein is composed of 85 kDa regulatory subunit and 110 kDa catalytic subunit. The most common mutations of PIK3CA occur in exon 9 and exon 20. H1047R, one of the most common mutations occurs in exon 20. PIK3CA plays an important role in the PI3K/AKT/mTOR signaling pathway, which can be activated by this mutation, closely related to the occurrence of tumors and disease progression. In non-small cell lung cancer, H1047R mutantion can phosphorylate Akt and activate downstream pathways to promote the survival and expansion of tumor cells.

Specifications

Size	50μL/100μL
Target	PIK3CA H1047R
Reactivity	Human
Applications	IHC, WB, IF, ELISA
MW(Calculated)	124kD
MW(Observed)	124kD
Host Species	Mouse
Isotype	IgG2b,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:100-500; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RMM454
Immunogen	Synthesized peptide derived from human PIK3CA H1047R AA range: 1000-1068
Specificity	The antibody can specifically recognize the h1047r mutation of human PIK3CA protein, and does not react with wild-type PIK3CA protein.
Gene Name	PIK3CA
Protein Name	5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; 5-bisphosphate 3-kinase catalytic subunit alpha isoform; caPI3K; CLOVE; CWS5; MCAP; MCM; MCMTC; MGC142161; MGC142163; p110 alpha; p110alpha; Phosphatidylinositol 3 kinase catalytic alpha polypeptide; Phosphatidylinositol 3 kinase catalytic 110 KD alpha; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha; Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform; Phosphatidylinositol 4,5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha; Phosphatidylinositol-4; Phosphoinositide 3 kinase catalytic alpha polypeptide; PI 3 Kinase catalytic subunit alpha; PI3 kinase p110 subunit alpha; PI3-kinase subunit alpha; PI3K; PI3K-alpha; PI3KC A; PIK3C A; Pik3ca; PK3CA; PK3CA_HUMAN; PtdIns 3 kinase p110; PtdIns-3-kinase subunit alpha; PtdIns-3-kinase subunit p110-alpha; Serine/threonine protein kinase PIK3CA
Other Name	5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; / 5-bisphosphate 3-kinase catalytic subunit alpha isoform; / caPI3K; / CLOVE; / CWS5; / MCAP; / MCM; / MCMTC; / MGC142161; / MGC142163; / p110 alpha; / p110alpha; / Phosphatidylinositol 3 kinase catalytic alpha polypeptide; / Phosphatidylinositol 3 kinase catalytic 110 KD alpha; / Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha; / Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform; / Phosphatidylinositol 4, 5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha; / Phosphatidylinositol-4; / Phosphoinositide 3 kinase catalytic alpha polypeptide; / PI 3 Kinase catalytic subunit alpha; / PI3 kinase p110 subunit alpha; / PI3-kinase subunit alpha; / PI3K; / PI3K-alpha; / PI3KC A; / PIK3C A; / Pik3ca; / PK3CA; / PK3CA_HUMAN; / PtdIns 3 kinase p110; / PtdIns-3-kinase subunit alpha; / PtdIns-3-kinase subunit p110-alpha; / Serine/threonine protein kinase PIK3CA
Species	Human
Gene ID	5290
Uniprot	P42336,
Species.1	Mouse
Gene ID.1	18706
Uniprot.1	P42337
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasmic
Tissue Expression	Gastric adenocarcinoma with PIK3CA H1047R protein expression
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cell growth and death >> Cellular senescence;Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> B cell receptor signaling pathway;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Organismal Systems >> Immune system >> Chemokine signaling pathway;Organismal Systems >> Endocrine system >> Insulin signaling pathway;Organismal Systems >> Endocrine system >> Estrogen signaling pathway;Organismal Systems >> Endocrine system >> Prolactin signaling pathway;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Organismal Systems >> Endocrine system >> Growth hormone synthesis, secretion and action;Organismal Systems >> Endocrine system >> Thyroid hormone signaling pathway;Organismal Systems >> Nervous system >> Cholinergic synapse;Organismal Systems >> Nervous system >> Neurotrophin signaling pathway;Organismal Systems >> Sensory system >> Inflammatory mediator regulation of TRP channels;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Organismal Systems >> Aging >> Longevity regulating pathway;Organismal Systems >> Aging >> Longevity regulating pathway - multiple species;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: overview >> Central carbon metabolism in cancer;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Pancreatic cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Cancer: specific types >> Glioma;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Human Diseases >> Cancer: specific types >> Chronic myeloid leukemia;Human Diseases >> Cancer: specific types >> Melanoma;Human Diseases >> Cancer: specific types >> Renal cell carcinoma;Human Diseases >> Cancer: specific types >> Prostate cancer;Human Diseases >> Cancer: specific types >> Endometrial cancer;Human Diseases >> Cancer: specific types >> Breast cancer;Human Diseases >> Cancer: specific types >> Small cell lung cancer;Human Diseases >> Cancer: specific types >> Non-small cell lung cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Spinocerebellar ataxia;Human Diseases >> Neurodegenerative disease >> Prion disease;Environmental Information Processing >> Signal transduction >> ErbB signaling pathway;Environmental Information Processing >> Signal transduction >> Ras signaling pathway;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway;Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> FoxO signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Research Areas	>>Inositol phosphate metabolism; / >>Metabolic pathways; / >>EGFR tyrosine kinase inhibitor resistance; / >>Endocrine resistance; / >>Platinum drug resistance; / >>ErbB signaling pathway; / >>Ras signaling pathway; / >>Rap1 signaling pathway; / >>cAMP signaling pathway; / >>Chemokine signaling pathway; / >>HIF-1 signaling pathway; / >>FoxO signaling pathway; / >>Phosphatidylinositol signaling system; / >>Sphingolipid signaling pathway; / >>Phospholipase D signaling pathway; / >>Autophagy - animal; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>AMPK signaling pathway; / >>Apoptosis; / >>Longevity regulating pathway; / >>Longevity regulating pathway - multiple species; / >>Cellular senescence; / >>Axon guidance; / >>VEGF signaling pathway; / >>Osteoclast differentiation; / >>Focal adhesion; / >>Signaling pathways regulating pluripotency of stem cells; / >>Platelet activation; / >>Neutrophil extracellular trap formation; / >>Toll-like receptor signaling pathway; / >>C-type lectin receptor signaling pathway; / >>JAK-STAT signaling pathway; / >>Natural killer cell mediated cytotoxicity; / >>T cell receptor signaling pathway; / >>B cell receptor signaling pathway; / >>Fc epsilon RI signaling pathway; / >>Fc gamma R-mediated phagocytosis; / >>TNF signaling pathway; / >>Leukocyte transendothelial migration; / >>Neurotrophin signaling pathway; / >>Cholinergic synapse; / >>Inflammatory mediator regulation of TRP channels; / >>Regulation of actin cytoskeleton; / >>Insulin signaling pathway; / >>Progesterone-mediated oocyte maturation; / >>Estrogen signaling pathway; / >>Prolactin signaling pathway; / >>Thyroid hormone signaling pathway; / >>Regulation of lipolysis in adipocytes; / >>Relaxin signaling pathway; / >>GnRH secretion; / >>Type II diabetes mellitus; / >>Insulin resistance; / >>Non-alcoholic fatty liver disease; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Growth hormone synthesis, secretion and action; / >>Aldosterone-regulated sodium reabsorption; / >>Carbohydrate digestion and absorption; / >>Alzheimer disease; / >>Spinocerebellar ataxia; / >>Prion disease; / >>Bacterial invasion of epithelial cells; / >>Shigellosis; / >>Salmonella infection; / >>Yersinia infection; / >>Chagas disease; / >>Amoebiasis; / >>Hepatitis C; / >>Hepatitis B; / >>Measles; / >>Human cytomegalovirus infection; / >>Influenza A; / >>Human papillomavirus infection; / >>Human T-cell leukemia virus 1 infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Herpes simplex virus 1 infection; / >>Epstein-Barr virus infection; / >>Human immunodeficiency virus 1 infection; / >>Coronavirus disease - COVID-19; / >>Pathways in cancer; / >>Viral carcinogenesis; / >>Proteoglycans in cancer; / >>MicroRNAs in cancer; / >>Chemical carcinogenesis - receptor activation; / >>Chemical carcinogenesis - reactive oxygen species; / >>Colorectal cancer; / >>Renal cell carcinoma; / >>Pancreatic cancer; / >>Endometrial cancer; / >>Glioma; / >>Prostate cancer; / >>Melanoma; / >>Chronic myeloid leukemia; / >>Acute myeloid leukemia; / >>Small cell lung cancer; / >>Non-small cell lung cancer; / >>Breast cancer; / >>Hepatocellular carcinoma; / >>Gastric cancer; / >>Central carbon metabolism in cancer; / >>Choline metabolism in cancer; / >>PD-L1 expression and PD-1 checkpoint pathway in cancer; / >>Diabetic cardiomyopathy; / >>Lipid and atherosclerosis; / >>Fluid shear stress and atherosclerosis
Function	Catalytic activity:ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate.,Disease:Defects in PIK3CA are associated with breast cancer [MIM:114480].,Disease:Defects in PIK3CA are associated with colorectal cancer (CRC) [MIM:114500].,Disease:Defects in PIK3CA are associated with ovarian cancer [MIM:167000]. Ovarian cancer is the leading cause of death from gynecologic malignancy. It is characterized by advanced presentation with loco-regional dissemination in the peritoneal cavity and the rare incidence of visceral metastases. These typical features relate to the biology of the disease, which is a principal determinant of outcome.,Disease:Defects in PIK3CA may underlie hepatocellular carcinoma (HCC) [MIM:114550].,Disease:PI3KCA mutations affecting exons 9 and 20 display gender-and tissue-specific patterns, thus suggesting that the different amino acid changes could exert distinct functional effects on the oncogenic properties of this enzyme. Furthermore, sexual dimorphisms and tissue specific factors might directly or indirectly influence the occurrence of PI3KCA cancer alleles.,Function:Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.,similarity:Belongs to the PI3/PI4-kinase family.,similarity:Contains 1 C2 domain.,similarity:Contains 1 PI3K/PI4K domain.,subunit:Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Binds to IRS1 in nuclear extracts. Interacts with RUFY3.,

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PIK3CA H1047R [ABT454] mouse mAb

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PIK3CA H1047R [ABT454] mouse mAb

Product details

Specifications

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