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Home/Reagents/Antibody/Primary antibody/Monoclonal Antibody/Rabbit mAb/U-Blot® Pim-1 Rabbit mAb
SKU: WR5000 Categories: ,

U-Blot® Pim-1 Rabbit mAb

Ucallm_WR5000-50

Price range: $268.00 through $328.00

SKU: WR5000-50
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Product details

Background:The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines, and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells, contributing to both cell proliferation and survival, and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons, the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805, 1825810).[provided by RefSeq, Aug 2011],

Specifications

Size50μL/100μL
TargetPim-1
ReactivityHuman, Mouse
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)45kD
MW(Observed)34kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM1232R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NamePIM1
Protein NameSerine/threonine-protein kinase pim-1
Other NamePIM1; / Serine/threonine-protein kinase pim-1
SpeciesHuman
Gene ID5292
UniprotP11309,
Species.1Mouse
Gene ID.1
Uniprot.1P06803,
Species.2Rat
Gene ID.224649
Uniprot.2P26794
Cellular Localization[Isoform 1]: Cytoplasm. Nucleus.; [Isoform 2]: Cell membrane.
Tissue ExpressionExpressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
Signaling_pathwayHuman Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Human Diseases >> Cancer: specific types >> Acute myeloid leukemia;Environmental Information Processing >> Signal transduction >> JAK-STAT signaling pathway
Research Areas>>JAK-STAT signaling pathway; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Pathways in cancer; / >>MicroRNAs in cancer; / >>Acute myeloid leukemia
FunctionCatalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Manganese.,Function:Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provide a selective advantage in tumorigenesis. May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3.,induction:Strongly induced in leukocytes by the JAK/STAT pathway in response to cytokines.,PTM:Autophosphorylated on both serine/threonine and tyrosine residues.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Binds to RP9. Isoform 2 is isolated as a monomer whereas isoform 1 complexes with other proteins. Isoform 1, but not isoform 2, binds BMX.,tissue specificity:Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.,
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