Price range: $268.00 through $328.00
Background:
The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2013],
| Size | 50μL/100μL |
| Target | Pirh2 |
| Reactivity | Human, Rat |
| Applications | WB, IHC, IF, FC, ELISA |
| MW(Calculated) | 30kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 5C9 |
| Immunogen | Purified recombinant fragment of human Pirh2 expressed in E. Coli. |
| Specificity | Pirh2 Monoclonal Antibody detects endogenous levels of Pirh2 protein. |
| Gene Name | RCHY1 |
| Protein Name | RING finger and CHY zinc finger domain-containing protein 1 |
| Other Name | RCHY1; / ARNIP; / CHIMP; / PIRH2; / RNF199; / ZNF363; / RING finger and CHY zinc finger domain-containing protein 1; / Androgen receptor N-terminal-interacting protein; / CH-rich-interacting match with PLAG1; / E3 ubiquitin-protein ligase Pirh2; / RING finger |
| Species | Human |
| Gene ID | 25898 |
| Uniprot | Q96PM5, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q9CR50 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus. Nucleus speckle. Cytoplasm. |
| Tissue Expression | Liver,Platelet,Skin,Uterus, |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> p53 signaling pathway;Genetic Information Processing >> Folding, sorting and degradation >> Ubiquitin mediated proteolysis |
| Research Areas | >>p53 signaling pathway; / >>Ubiquitin mediated proteolysis; / >>Measles |
| Function | similarity:Contains 1 CHY-type zinc finger.,similarity:Contains 1 CTCHY-type zinc finger.,similarity:Contains 1 RING-type zinc finger.,subunit:Interacts with AR, PLAG1 and SCYL1BP1/NTKLBP1., |
