Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a member of the RAS superfamily of small GTPases. The encoded protein is involved in membrane trafficking and cell survival. This gene has been found to be a tumor suppressor and an oncogene, depending on the context. Two variants, one protein-coding and the other not, have been found for this gene. [provided by RefSeq, Nov 2015],
| Size | 50μL/100μL |
| Target | Rab25 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, FC, ELISA |
| MW(Calculated) | 23kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:200-1:1000; IF 1:200-1:1000; Flow Cyt 1:200-1:400; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 5A7 |
| Immunogen | Purified recombinant fragment of Rab 25 expressed in E. Coli. |
| Specificity | Rab 25 Monoclonal Antibody detects endogenous levels of Rab 25 protein. |
| Gene Name | RAB25 CATX8 |
| Protein Name | Ras-related protein Rab-25 |
| Other Name | RAB25; / CATX8; / Ras-related protein Rab-25; / CATX-8 |
| Species | Human |
| Gene ID | 57111 |
| Uniprot | P57735, |
| Species.1 | Mouse |
| Gene ID.1 | 53868 |
| Uniprot.1 | Q9WTL2 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell membrane; Lipid-anchor; Cytoplasmic side . Cell projection, pseudopodium membrane . Cytoplasmic vesicle . Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips. . |
| Tissue Expression | Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:May selectively regulate the apical recycling and/or transcytotic pathways.,similarity:Belongs to the small GTPase superfamily. Rab family.,subunit:Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4., |
