SKU: WN0243 Categories: ,

Recombinant CD276/B7H3 Fc-Fused Nanobody (VF0586)

$365.00

SKU: WN0243
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Product details

Background:The protein encoded byThis gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript ofThis gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR ofThis transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression ofThis protein and miR29 levels, suggesting regulation of expression ofThis gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found forThis gene. [provided by RefSeq, Sep 2011]

Specifications

Size100μL
TargetCD276/B7H3
ReactivityHuman
ApplicationsELISA
MW(Calculated)
MW(Observed)
Host Species
IsotypeIgG2a,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioELISA 1:5000-100000
FormulationPhosphate-buffered solution
PurificationRecombinant Expression and Affinity purified
Storage-15°C to -25°C/1 year(Avoid freeze / thaw cycles)
ConcentrationPlease check the information on the tube
Clonality
Clone NumberVF0395
ImmunogenPurified recombinant Human CD276
SpecificityThis recombinant monoclonal antibody can detects endogenous levels of CD276/B7H3 protein.
Gene NameCD276 B7H3 PSEC0249 UNQ309/PRO352
Protein NameCD276 antigen (4Ig-B7-H3) (B7 homolog 3) (B7-H3) (Costimulatory molecule) (CD antigen CD276)
Other NameCD276; / B7H3; / PSEC0249; / CD276 antigen; / 4Ig-B7-H3; / B7 homolog 3; / B7-H3; / Costimulatory molecule; / CD antigen CD276; / CD276 nanobody; / B7H3 nanobody
SpeciesHuman
Gene ID80381
UniprotQ5ZPR3
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationMembrane; Single-pass type I membrane protein .
Tissue ExpressionExpressed in B-cell lines and T-lymphocytes.
Signaling_pathwayEnvironmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules
Research Areas
FunctionMay participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.,induction:By LPS in monocytes and by ionomycin in T and B lymphocytes. Up-regulated in cells mediating rejection of human transplants.,miscellaneous:B7-H3 locus underwent genomic duplication leading to tandemly repeated immunoglobulin-like V and C domains (VC domains). The dominantly expressed human B7-H3 isoform contains tandemly duplicated VC domains. In contrast, mouse B7-H3 transcript contains only one single VC domain form due to an exon structure corresponding to V domain-(pseudoexon C)-(pseudoexon V)-C domain. This duplication appearing in primates is suggested to be very recent supporting a model of multiple independent emergence of tandem VC repeats within human and monkey species.,similarity:Belongs to the immunoglobulin superfamily. BTN/MOG family.,similarity:Contains 2 Ig-like C2-type (immunoglobulin-like) domains.,similarity:Contains 2 Ig-like V-type (immunoglobulin-like) domains.,subunit:Interacts with TREML2 and this interaction enhances T-cell activation.,tissue specificity:Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.,
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