Name: Recombinant GFP Fc-Fused Nanobody (VF0481)
SKU: WN0588
Price: 365 USD
Availability: InStock

Recombinant GFP Fc-Fused Nanobody (VF0481)

Ucallm_WN0588

$365.00

Product details

Background:Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.

Specifications

Size	100μL
Target	GFP
Reactivity	Human
Applications	ELISA
MW(Calculated)
MW(Observed)
Host Species
Isotype	IgG2a,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	ELISA 1:5000-100000; Flow Cyt 1-2μg/Test
Formulation	Phosphate-buffered solution
Purification	Recombinant Expression and Affinity purified
Storage	-15°C to -25°C/1 year(Avoid freeze / thaw cycles)
Concentration	Please check the information on the tube
Clonality
Clone Number	VF0186
Immunogen	Purified recombinant GFP
Specificity	This recombinant monoclonal antibody can detects endogenous levels of GFP protein.
Gene Name	CD4
Protein Name	T-cell surface glycoprotein CD4 (T-cell surface antigen T4/Leu-3) (CD antigen CD4)
Other Name	GFP nanobody
Species	Human
Gene ID
Uniprot
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cell membrane; Single-pass type I membrane protein . Localizes to lipid rafts (PubMed:12517957, PubMed:9168119). Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.
Tissue Expression	Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells.
Signaling_pathway
Research Areas
Function	Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.; (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) . Down-regulated by HIV-1 Vpu . Acts as a receptor for Human Herpes virus 7/HHV-7 .