SKU: WN0589 Categories: ,

Recombinant GFP Fc-Fused Nanobody (VF0482)

$365.00

SKU: WN0589
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Product details

Background:Green Fluorescent Protein (GFP) has quickly become a powerful research tool for assessing gene expression and subcellular protein distribution in fixed or living cells. GFP is excited by and brightly fluoresces when exposed to UV or blue light. This feature makes it ideal as a marker for use in fluorescence microscopy, cytometry, tagging fusion proteins, and assaying transcriptional regulation from gene promoters in vivo. Numerous GFP variants with enhanced and shifted emission spectra (blue, green, and yellow) have been developed through amino acid substitutions at specific residues.

Specifications

Size100μL
TargetGFP
ReactivityHuman
ApplicationsELISA
MW(Calculated)
MW(Observed)
Host Species
IsotypeIgG2a,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioELISA 1:5000-100000; Flow Cyt 1-2μg/Test
FormulationPhosphate-buffered solution
PurificationRecombinant Expression and Affinity purified
Storage-15°C to -25°C/1 year(Avoid freeze / thaw cycles)
ConcentrationPlease check the information on the tube
Clonality
Clone NumberVF0138
ImmunogenPurified recombinant GFP
SpecificityThis recombinant monoclonal antibody can detects endogenous levels of GFP protein.
Gene NameSELL LNHR LYAM1
Protein NameL-selectin (CD62 antigen-like family member L) (Leukocyte adhesion molecule 1) (LAM-1) (Leukocyte surface antigen Leu-8) (Leukocyte-endothelial cell adhesion molecule 1) (LECAM1) (Lymph node homing receptor) (TQ1) (gp90-MEL) (CD antigen CD62L)
Other NameGFP nanobody
SpeciesHuman
Gene ID
Uniprot
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationCell membrane; Single-pass type I membrane protein .
Tissue ExpressionExpressed in B-cell lines and T-lymphocytes.
Signaling_pathway
Research Areas
FunctionCalcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells (PubMed:12403782, PubMed:28489325, PubMed:28011641).Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia (PubMed:12403782, PubMed:28011641).
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