Name: Recombinant TNF-α Fc-Fused Nanobody (VF0465)
SKU: WN0653
Price: 365 USD
Availability: InStock

Product details

Background:This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008],

Specifications

Target	TNFA
Reactivity	Human
Applications	ELISA
MW(Calculated)
MW(Observed)
Host Species
Isotype	IgG2a,Kappa
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	ELISA 1:5000-100000
Formulation	Phosphate-buffered solution
Source	Camel, chimeric fusion of Nanobody (VHH) and mouse IgG1 Fc domain , recombinantly produced from 293F cell
Purification	Recombinant Expression and Affinity purified
Purity
Storage	-15°C to -25°C/1 year(Avoid freeze / thaw cycles)
Concentration	Please check the information on the tube
Clonality
Clone Number	PN0064
Immunogen	Purified recombinant Human TNFa
Sequence
Specificity	This recombinant monoclonal antibody can detects endogenous levels of TNFa protein.
Gene Name	TNF TNFA TNFSF2
Protein Name	Tumor necrosis factor (Cachectin) (TNF-alpha) (Tumor necrosis factor ligand superfamily member 2) (TNF-a)
Other Name	TNF; / TNFA; / TNFSF2; / Tumor necrosis factor; / Cachectin; / TNF-alpha; / Tumor necrosis factor ligand superfamily member 2; / TNF-a; / TNFa nanobody
Species	Human
Gene ID-1	7124
Uniprot	P01375
Species.1
Gene ID-2
Uniprot.1
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008],
Cellular Localization	Cell membrane; Single-pass type II membrane protein .; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted .; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
Tissue Expression	Expressed in some types of immune cells. Expressed at low levels on the surface of freshly isolated T-cells and natural killer (NK) cells, predominantly on CD8+ T-cells (mainly memory/effector, but not naive cells) and on both CD16+ and CD16- NK cells. T-cell expression levels are variable among individuals. Not detected in B-cells, naive or helper T-cells, monocytes, nor neutrophils (at protein level). Not detected in dendritic cells.
Signaling_pathway	Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> Hematopoietic cell lineage;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> RIG-I-like receptor signaling pathway;Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Organismal Systems >> Immune system >> Antigen processing and presentation;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> IL-17 signaling pathway;Organismal Systems >> Immune system >> Fc epsilon RI signaling pathway;Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Immune disease >> Asthma;Human Diseases >> Immune disease >> Systemic lupus erythematosus;Human Diseases >> Immune disease >> Rheumatoid arthritis;Human Diseases >> Immune disease >> Inflammatory bowel disease;Human Diseases >> Immune disease >> Allograft rejection;Human Diseases >> Immune disease >> Graft-versus-host disease;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> TGF-beta signaling pathway;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> Cytokine-cytokine receptor interaction;Environmental Information Processing >> Signaling molecules and interaction >> Viral protein interaction with cytokine and cytokine receptor
Research Areas
Function	Disease:Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition.,Disease:Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.,Disease:Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis.,Function:Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.,online information:The Singapore human mutation and polymorphism database,online information:Tumor necrosis factor alpha entry,PTM:O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.,PTM:The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.,PTM:The soluble form derives from the membrane form by proteolytic processing.,similarity:Belongs to the tumor necrosis factor family.,subunit:Homotrimer.,
RRID

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Recombinant TNF-α Fc-Fused Nanobody (VF0465)

Product details

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Recombinant TNF-α Fc-Fused Nanobody (VF0465)

Product details

Specifications

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