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Home/Reagents/Antibody/Primary antibody/Polyclonal Antibody/Rabbit pAb/RFC1 Polyclonal Antibody
SKU: WP15429 Categories: ,

RFC1 Polyclonal Antibody

Ucallm_WP15429-50

Price range: $268.00 through $328.00

SKU: WP15429-100
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Product details

Background:

This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011],

Specifications

Size50μL/100μL
TargetRFC1
ReactivityHuman, Mouse
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)130kD
Host SpeciesRabbit
IsotypeIgG
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC: 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from the C-terminal region of human RFC1. AA range:1071-1120
SpecificityRFC1 Polyclonal Antibody detects endogenous levels of RFC1 protein.
Gene NameRFC1
Protein NameReplication factor C subunit 1
Other NameRFC1; / RFC140; / Replication factor C subunit 1; / Activator 1 140 kDa subunit; / A1 140 kDa subunit; / Activator 1 large subunit; / Activator 1 subunit 1; / DNA-binding protein PO-GAReplication factor C 140 kDa subunit; / RF-C 140 kDa subunit; / RFC140; / Replication factor C large subunit
SpeciesHuman
Gene ID5981
UniprotP35251,
Species.1Mouse
Gene ID.119687
Uniprot.1P35601
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationNucleus.
Tissue ExpressionWide tissue distribution. Undetectable in placental tissue.
Signaling_pathway
Research Areas>>DNA replication; / >>Nucleotide excision repair; / >>Mismatch repair
FunctionFunction:Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.,Function:The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the activator 1 large subunit family.,similarity:Contains 1 BRCT domain.,subunit:Heterotetramer of subunits RFC2, RFC3, RFC4 and RFC5 that can form a complex either with RFC1 or with RAD17. The former interacts with PCNA in the presence of ATP, while the latter has ATPase activity but is not stimulated by PCNA.,tissue specificity:Wide tissue distribution. Undetectable in placental tissue.,
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