Price range: $268.00 through $328.00
Background:
ring finger protein 138(RNF138) Homo sapiens The protein encoded by this gene contains a RING finger, a motif present in a variety of functionally distinct proteins and known to be involved in protein-DNA and protein-protein interactions. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | RNF138 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| MW(Calculated) | |
| MW(Observed) | 28kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-300 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RNF138. AA range:31-80 |
| Specificity | RNF138 Polyclonal Antibody detects endogenous levels of RNF138 protein. |
| Gene Name | RNF138 |
| Protein Name | E3 ubiquitin-protein ligase RNF138 |
| Other Name | RNF138; / NARF; / HSD-4; / HSD4; / E3 ubiquitin-protein ligase RNF138; / Nemo-like kinase-associated RING finger protein; / NLK-associated RING finger protein; / hNARF; / RING finger protein 138 |
| Species | Human |
| Gene ID | 51444 |
| Uniprot | Q8WVD3, |
| Species.1 | Mouse |
| Gene ID.1 | 56515 |
| Uniprot.1 | Q9CQE0, |
| Species.2 | Rat |
| Gene ID.2 | 94196 |
| Uniprot.2 | Q99PD2 |
| Cellular Localization | Chromosome . Recruited at DNA damage sites (PubMed:26502055). Localizes to sites of double-strand break: localization to double-strand break sites is mediated by the zinc fingers (PubMed:26502055, PubMed:26502057). . |
| Tissue Expression | Placenta,Testis,Uterus, |
| Signaling_pathway | |
| Research Areas | |
| Function | Domain:The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.,Function:E3 ubiquitin-protein ligase. Together with NLK, involved in the ubiquitination and degradation of TCF/LEF. Also exhibits auto-ubiquitination activity in combination with UBE2K. May act as a negative regulator in the Wnt/beta-catenin-mediated signaling pathway.,pathway:Protein modification; protein ubiquitination.,PTM:Auto-ubiquitinated.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 RING-type zinc finger.,subunit:Interacts with nlk., |
