Price range: $268.00 through $328.00
Background:
Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008],
| Size | 50μL/100μL |
| Target | RPA135 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 125kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from the Internal region of human RPA135. |
| Specificity | RPA135 Polyclonal Antibody detects endogenous levels of RPA135 protein. |
| Gene Name | POLR1B |
| Protein Name | DNA-directed RNA polymerase I subunit RPA2 |
| Other Name | POLR1B; / DNA-directed RNA polymerase I subunit RPA2; / RNA polymerase I subunit 2; / DNA-directed RNA polymerase I 135 kDa polypeptide; / RPA135 |
| Species | Human |
| Gene ID | 84172 |
| Uniprot | Q9H9Y6, |
| Species.1 | Mouse |
| Gene ID.1 | 20017 |
| Uniprot.1 | P70700, |
| Species.2 | Rat |
| Gene ID.2 | 83582 |
| Uniprot.2 | O54888 |
| Cellular Localization | Nucleus, nucleolus . Chromosome . |
| Tissue Expression | Eye,Pancreas,Skin,Testis, |
| Signaling_pathway | |
| Research Areas | >>RNA polymerase |
| Function | Catalytic activity:Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).,Function:DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.,similarity:Belongs to the RNA polymerase beta chain family.,subunit:Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits., |
