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U-Blot® RPS6 Rabbit mAb
WR4456 3T3-L1 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
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Product details
Background:Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed
Specifications
| Size | 50μL/100μL |
| Target | Ribosomal Protein S6 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 29kD |
| MW(Observed) | 32kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:1000-1:5000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0676R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | RPS6 |
| Protein Name | 40S ribosomal protein S6 |
| Other Name | RPS6; / OK/SW-cl.2; / 40S ribosomal protein S6; / Phosphoprotein NP33 |
| Species | Human |
| Gene ID | 6194 |
| Uniprot | P62753, |
| Species.1 | Mouse |
| Gene ID.1 | 20104 |
| Uniprot.1 | P62754, |
| Species.2 | Rat |
| Gene ID.2 | 29304 |
| Uniprot.2 | P62755 |
| Cellular Localization | Cytoplasm,Nucleus |
| Tissue Expression | Brain,Colon,Colon adenocarcinoma,Epithelium,Muscle,Ovary,Pancreas,Placenta,Skin,Tes |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Insulin signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> HIF-1 signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway |
| Research Areas | >>EGFR tyrosine kinase inhibitor resistance; / >>Ribosome; / >>HIF-1 signaling pathway; / >>mTOR signaling pathway; / >>PI3K-Akt signaling pathway; / >>Apelin signaling pathway; / >>Thermogenesis; / >>Insulin signaling pathway; / >>Coronavirus disease - COVID-19; / >>Proteoglycans in cancer |
| Function | Function:May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.,PTM:Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest.,similarity:Belongs to the ribosomal protein S6e family., |
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