Name: RyR-2 Polyclonal Antibody
SKU: WP14386
Price: 268 USD
Availability: InStock

Product details

Background:

This gene encodes a ryanodine receptor found in cardiac muscle sarcoplasmic reticulum. The encoded protein is one of the components of a calcium channel, composed of a tetramer of the ryanodine receptor proteins and a tetramer of FK506 binding protein 1B proteins, that supplies calcium to cardiac muscle. Mutations in this gene are associated with stress-induced polymorphic ventricular tachycardia and arrhythmogenic right ventricular dysplasia. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	RyR-2
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)
MW(Observed)	200-300kD
Host Species	Rabbit
Isotype	IgG
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration	1 mg/ml
Clonality	Polyclonal
Clone Number
Immunogen	The antiserum was produced against synthesized peptide derived from human RyR2. AA range:2774-2823
Specificity	RyR-2 Polyclonal Antibody detects endogenous levels of RyR-2 protein.
Gene Name	RYR2
Protein Name	Ryanodine receptor 2
Other Name	RYR2; / Ryanodine receptor 2; / RYR-2; / RyR2; / hRYR-2; / Cardiac muscle ryanodine receptor; / Cardiac muscle ryanodine receptor-calcium release channel; / Type 2 ryanodine receptor
Species	Human
Gene ID	6262
Uniprot	Q92736,
Species.1	Mouse
Gene ID.1	20191
Uniprot.1	E9Q401,
Species.2	Rat
Gene ID.2	689560
Uniprot.2	B0LPN4
Cellular Localization	Sarcoplasmic reticulum membrane; Multi-pass membrane protein . Membrane; Multi-pass membrane protein . Sarcoplasmic reticulum . The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic. .
Tissue Expression	Detected in heart muscle (at protein level). Heart muscle, brain (cerebellum and hippocampus) and placenta.
Signaling_pathway	Organismal Systems >> Endocrine system >> Insulin secretion;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Circulatory system >> Cardiac muscle contraction;Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway
Research Areas	>>Calcium signaling pathway; / >>cAMP signaling pathway; / >>Cardiac muscle contraction; / >>Adrenergic signaling in cardiomyocytes; / >>Apelin signaling pathway; / >>Circadian entrainment; / >>Insulin secretion; / >>Oxytocin signaling pathway; / >>Pancreatic secretion; / >>Prion disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Hypertrophic cardiomyopathy; / >>Arrhythmogenic right ventricular cardiomyopathy; / >>Dilated cardiomyopathy; / >>Diabetic cardiomyopathy
Function	developmental stage:Expressed in myometrium during pregnancy.,Disease:Defects in RYR2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 1 (CPVT1) [MIM:604772]; also known as stress-induced polymorphic ventricular tachycardia (VTSIP). CPVT1 is an autosomal dominant form of arrhythmogenic disorder characterized by stress-induced, bidirectional ventricular tachycardia that may degenerate into cardiac arrest and cause sudden death.,Disease:Defects in RYR2 are the cause of familial arrhythmogenic right ventricular dysplasia 2 (ARVD2) [MIM:600996]; also known as arrhythmogenic right ventricular cardiomyopathy 2 (ARVC2). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall.,Function:Communication between transverse-tubules and sarcoplasmic reticulum. Contraction of cardiac muscle is triggered by release of calcium ions from SR following depolarization of T-tubules.,induction:By TGF-beta.,miscellaneous:Ryanodine is an alkaloid that binds to the Ca-release channel in junctional SR and modulates its activity.,miscellaneous:The calcium release channel activity resides in the C-terminal region while the remaining part of the protein constitutes the 'foot' structure spanning the junctional gap between the SR and the T-tubule. It is possible that the foot structure interacts with the cytoplasmic region of the dihydropyridine receptor.,miscellaneous:The calcium release channel is modulated by calcium ions, magnesium ions, ATP and calmodulin.,online information:Ryanodine receptor entry,online information:RYR2 entry,similarity:Belongs to the ryanodine receptor family.,similarity:Contains 2 EF-hand domains.,similarity:Contains 3 B30.2/SPRY domains.,similarity:Contains 5 MIR domains.,subunit:Homotetramer .,tissue specificity:Heart muscle, brain (cerebellum and hippocampus) and placenta.,