U-Blot® SMAD5 (Phospho Ser463/Ser465) Rabbit mAb
WR4531 Western Blot analysis using HeLa lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(WS0446)was diluted at 1:10000, 37° 1hour.
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Product details
Background:The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014],
Specifications
| Size | 50μL/100μL |
| Target | Smad5 Phospho Ser463/465 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 52kD |
| MW(Observed) | 60kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | phosphate |
| Recommended Dilution Ratio | WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000-1:20000; |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0663R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | SMAD5 |
| Protein Name | Mothers against decapentaplegic homolog 5 |
| Other Name | SMAD5; / MADH5; / Mothers against decapentaplegic homolog 5; / MAD homolog 5; / Mothers against DPP homolog 5; / JV5-1; / SMAD family member 5; / SMAD 5; / Smad5; / hSmad5 |
| Species | Human |
| Gene ID | 4087 |
| Uniprot | Q99717, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P97454, |
| Species.2 | Rat |
| Gene ID.2 | 29357 |
| Uniprot.2 | O70436 |
| Cellular Localization | Cytoplasm,Nucleus |
| Tissue Expression | Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta. |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Cell cycle;Cellular Processes >> Cell growth and death >> Cellular senescence;Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells;Organismal Systems >> Immune system >> Th17 cell differentiation;Organismal Systems >> Endocrine system >> Relaxin signaling pathway;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Colorectal cancer;Human Diseases >> Cancer: specific types >> Pancreatic cancer;Human Diseases >> Cancer: specific types >> Hepatocellular carcinoma;Human Diseases >> Cancer: specific types >> Gastric cancer;Human Diseases >> Immune disease >> Inflammatory bowel disease;Environmental Information Processing >> Signal transduction >> TGF-beta signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway |
| Research Areas | >>Cell cycle; / >>Endocytosis; / >>Cellular senescence; / >>TGF-beta signaling pathway; / >>Apelin signaling pathway; / >>Hippo signaling pathway; / >>Signaling pathways regulating pluripotency of stem cells; / >>Th17 cell differentiation; / >>Relaxin signaling pathway; / >>AGE-RAGE signaling pathway in diabetic complications; / >>Chagas disease; / >>Human T-cell leukemia virus 1 infection; / >>Pathways in cancer; / >>Proteoglycans in cancer; / >>Colorectal cancer; / >>Pancreatic cancer; / >>Hepatocellular carcinoma; / >>Gastric cancer; / >>Inflammatory bowel disease; / >>Diabetic cardiomyopathy |
| Function | Function:Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).,PTM:Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.,PTM:Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.,similarity:Belongs to the dwarfin/SMAD family.,similarity:Contains 1 MH1 (MAD homology 1) domain.,similarity:Contains 1 MH2 (MAD homology 2) domain.,subcellular location:Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.,subunit:May form trimers with the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit and SMURF1. Interacts with SUV39H1 and SUV39H2.,tissue specificity:Ubiquitous., |
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