Price range: $268.00 through $328.00
Background:
This gene is a member of the SMCY homolog family and encodes a protein with one ARID domain, one JmjC domain, one JmjN domain and two PHD-type zinc fingers. The DNA-binding motifs suggest this protein is involved in the regulation of transcription and chromatin remodeling. Mutations in this gene have been associated with X-linked mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009],
| Size | 50μL/100μL |
| Target | SMCX |
| Reactivity | Human, Mouse, Rat, Bovine, Dog |
| Applications | WB, IF |
| MW(Calculated) | 176kD |
| MW(Observed) | |
| Host Species | Mouse |
| Isotype | |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000-1:2000; IF 1:100-1:500; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | Affinity purification |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | 10A4 |
| Immunogen | Purified recombinant human SmcX (C-terminus) protein fragments expressed in E.coli. |
| Specificity | SmcX Monoclonal Antibody detects endogenous levels of SmcX protein. |
| Gene Name | KDM5C |
| Protein Name | Lysine-specific demethylase 5C |
| Other Name | KDM5C; / DXS1272E; / JARID1C; / SMCX; / XE169; / Lysine-specific demethylase 5C; / Histone demethylase JARID1C; / Jumonji/ARID domain-containing protein 1C; / Protein SmcX; / Protein Xe169 |
| Species | Human |
| Gene ID | 8242 |
| Uniprot | P41229, |
| Species.1 | Mouse |
| Gene ID.1 | 20591 |
| Uniprot.1 | P41230 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . |
| Tissue Expression | Expressed in all tissues examined. Highest levels found in brain and skeletal muscle. |
| Signaling_pathway | |
| Research Areas | |
| Function | cofactor:Alpha-ketoglutarate.,cofactor:Fe(2+).,Disease:Defects in KDM5C are a cause of X-linked mental retardation (XLMR) [MIM:300534]. Mental retardation is usually defined as cognitive impairment with an IQ less than 70. Etiologically, mental retardation is a very heterogeneous condition that involves environmental, stochastic and/or genetic factors.,Domain:Both the JmjC domain and the JmjN domain are required for enzymatic activity.,Domain:The first PHD-type zinc finger domain recognizes and binds H3-K9Me3.,Function:Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Participates in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements.,miscellaneous:Escapes X-inactivation.,similarity:Belongs to the JARID1 histone demethylase family.,similarity:Contains 1 ARID domain.,similarity:Contains 1 JmjC domain.,similarity:Contains 1 JmjN domain.,similarity:Contains 2 PHD-type zinc fingers.,subunit:Part of two distinct complexes, one containing E2F6, and the other containing REST.,tissue specificity:Expressed in all tissues examined. Highest levels found in brain and skeletal muscle., |
