SphK2 Polyclonal Antibody
Ucallm_WP14555-50
Price range: $268.00 through $328.00
Product details
Background:
This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011],
Specifications
| Size | 50μL/100μL |
| Target | SphK2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 70kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SPHK2. AA range:580-629 |
| Specificity | SphK2 Polyclonal Antibody detects endogenous levels of SphK2 protein. |
| Gene Name | SPHK2 |
| Protein Name | Sphingosine kinase 2 |
| Other Name | SPHK2; / Sphingosine kinase 2; / SK 2; / SPK 2 |
| Species | Human |
| Gene ID | 56848 |
| Uniprot | Q9NRA0, |
| Species.1 | Mouse |
| Gene ID.1 | 56632 |
| Uniprot.1 | Q9JIA7 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Nucleus . Endoplasmic reticulum . Mitochondrion inner membrane . In nucleus, located in nucleosomes where it associates with core histone proteins such as histone 3 (PubMed:19729656). In brains of patients with Alzheimer's disease, may be preferentially localized in the nucleus. Cytosolic expression decrease correlates with the density of amyloid deposits (PubMed:29615132). In apoptotic cells, colocalizes with CASP1 in cell membrane where is cleaved and released from cells in an active form (PubMed:20197547). .; [Isoform 2]: Lysosome membrane . |
| Tissue Expression | Mainly expressed in adult kidney, liver, and brain (PubMed:10751414). Expressed in cerebral cortex and hippocampus (at protein level) (PubMed:29615132). Isoform 1 is the predominant form expressed in most tissues (PubMed:16103110). |
| Signaling_pathway | Organismal Systems >> Immune system >> Fc gamma R-mediated phagocytosis;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway;Environmental Information Processing >> Signal transduction >> Apelin signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway |
| Research Areas | >>Sphingolipid metabolism; / >>Metabolic pathways; / >>Calcium signaling pathway; / >>Sphingolipid signaling pathway; / >>Phospholipase D signaling pathway; / >>VEGF signaling pathway; / >>Apelin signaling pathway; / >>Fc gamma R-mediated phagocytosis; / >>Tuberculosis |
| Function | Alternative products:Experimental confirmation may be lacking for some isoforms,Catalytic activity:ATP + sphinganine = ADP + sphinganine 1-phosphate.,Catalytic activity:ATP + sphingosine = ADP + sphingosine 1-phosphate.,cofactor:Magnesium.,Function:Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine.,similarity:Contains 1 DAGKc domain., |
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