Price range: $268.00 through $328.00
Background:
This gene encodes a member of the a ubiquitously expressed transcription factor that controls cholesterol homeostasis by regulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain and binds the sterol regulatory element 1 motif. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],
| Size | 50μL/100μL |
| Target | SREBP-2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 125kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human protein . at AA range: 390-470 |
| Specificity | SRBP2 Polyclonal Antibody detects endogenous levels of protein. |
| Gene Name | SREBF2 BHLHD2 SREBP2 |
| Protein Name | Sterol regulatory element-binding protein 2 (SREBP-2) (Class D basic helix-loop-helix protein 2) (bHLHd2) (Sterol regulatory element-binding transcription factor 2) [Cleaved into: Processed sterol regulatory element-binding protein 2] |
| Other Name | SRBP2; / Sterol regulatory element-binding protein 2; / SREBP-2; / Class D basic helix-loop-helix protein 2; / bHLHd2; / Sterol regulatory element-binding transcription factor 2; / [Cleaved into: Processed sterol regulatory element-binding protein 2] |
| Species | Human |
| Gene ID | 6721 |
| Uniprot | Q12772, |
| Species.1 | Mouse |
| Gene ID.1 | 20788 |
| Uniprot.1 | Q3U1N2, |
| Species.2 | Rat |
| Gene ID.2 | 300095 |
| Uniprot.2 | Q3T1I5 |
| Cellular Localization | [Sterol regulatory element-binding protein 2]: Endoplasmic reticulum membrane; Multi-pass membrane protein . Golgi apparatus membrane; Multi-pass membrane protein . Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein . At high sterol concentrations, the SCAP-SREBP is retained in the endoplasmic reticulum (PubMed:32322062). Low sterol concentrations promote recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi, where it is processed (PubMed:32322062). .; [Processed sterol regulatory element-binding protein 2]: Nucleus . Transported into the nucleus with the help of importin-beta. Dimerization of the bHLH domain is a prerequisite for importin beta-dependent nuclear import. . |
| Tissue Expression | Ubiquitously expressed in adult and fetal tissues. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.,PTM:At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.,similarity:Belongs to the SREBP family.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subcellular location:Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.,subunit:Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA.,tissue specificity:Ubiquitously expressed in adult and fetal tissues., |
