U-Blot® STAT5A Rabbit mAb
WR4110 Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
WR4110 Immunohistochemical analysis of paraffin-embedded rat spleen tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
WR4110 Immunohistochemical analysis of paraffin-embedded mouse spleen tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
WR4110 Immunohistochemical analysis of paraffin-embedded human tonsil tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
WR4110 Western blot analysis of lysates from Jurkat cells, using Ctip2 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG antibody (WS0002) was used to detect the antibody.
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Product details
Background:The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been
Specifications
| Size | 50μL/100μL |
| Target | Stat5A |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 91kD |
| MW(Observed) | 91kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0860R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | STAT5A |
| Protein Name | Signal transducer and activator of transcription 5A |
| Other Name | STAT5A; / STAT5; / Signal transducer and activator of transcription 5A |
| Species | Human |
| Gene ID | 64919 |
| Uniprot | P42229, |
| Species.1 | Mouse |
| Gene ID.1 | 20850 |
| Uniprot.1 | P42230, |
| Species.2 | Rat |
| Gene ID.2 | 24918 |
| Uniprot.2 | Q62771 |
| Cellular Localization | Cytoplasm, Nucleus |
| Tissue Expression | Brain,Cervix,Epithelium,Lung,Placenta,Synovial membrane |
| Signaling_pathway | Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer |
| Research Areas | >>Transcriptional misregulation in cancer |
| Function | Function:Carries out a dual Function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.,online information:STAT5 entry,PTM:Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.,similarity:Belongs to the transcription factor STAT family.,similarity:Contains 1 SH2 domain.,subcellular location:Translocated into the nucleus in response to phosphorylation.,subunit:Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (By similarity). Interacts with NCOA1 and SOCS7., |
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