SKU: WP9352 Categories: ,

Survivin (phospho Thr34) Polyclonal Antibody

$499.00

SKU: WP9352-100
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Product details

Background:

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors, yet low in adult tissues. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2011],

Specifications

TargetSurvivin
ReactivityHuman, Mouse, Rat
ApplicationsWB, ELISA, IHC
MW(Calculated)
MW(Observed)16kD
Host SpeciesRabbit
IsotypeIgG
Conjugate
ModificationPhospho
Recommended Dilution RatioWB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000
CompositionLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Source
Purification ProcessThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
Storage Condition-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration1 mg/ml
ClonalityPolyclonal
Clone Number
ImmunogenThe antiserum was produced against synthesized peptide derived from human Survivin around the phosphorylation site of Thr34. AA range:11-60
Sequence
SpecificityPhospho-Survivin (T34) Polyclonal Antibody detects endogenous levels of Survivin protein only when phosphorylated at T34.
Gene NameBIRC5
Protein NameBaculoviral IAP repeat-containing protein 5
AliasBIRC5;API4;IAP4;Baculoviral IAP repeat-containing protein 5;Apoptosis inhibitor 4;Apoptosis inhibitor survivin
Organism-1Human
Gene ID-1332
SwissProt-1O15392
Organism-2Mouse
Gene ID-211799
SwissProt-2O70201
Organism-3Rat
Gene ID-364041
SwissProt-3Q9JHY7
Organism-4
Gene ID-4
SwissProt-4
Cellular LocalizationCytoplasm . Nucleus . Chromosome . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle . Chromosome, centromere, kinetochore . Midbody . Localizes at the centromeres from prophase to metaphase, at the spindle midzone during anaphase and a the midbody during telophase and cytokinesis. Accumulates in the nucleus upon treatment with leptomycin B (LMB), a XPO1/CRM1 nuclear export inhibitor (By similarity). Localizes on chromosome arms and inner centromeres from prophase through metaphase. Localizes to kinetochores in metaphase, distributes to the midzone microtubules in anaphase and at telophase, localizes exclusively to the midbody (PubMed:11084331). Colocalizes with AURKB at mitotic chromosomes (PubMed:14610074). Acetylation at Lys-129 directs its localization to the nucleus by enhancing homodimerization and thereby inhibiting XPO1/CRM1-mediated nuclear export (PubMed:20826784). .
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