Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a nuclear outer membrane protein that binds cytoplasmic F-actin. This binding tethers the nucleus to the cytoskeleton and aids in the maintenance of the structural integrity of the nucleus. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009],
| Size | 50μL/100μL |
| Target | SYNE2 |
| Reactivity | Human, Mouse |
| Applications | IHC, IF |
| MW(Calculated) | |
| MW(Observed) | 757kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:50-300; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human protein . at AA range: 360-440 |
| Specificity | SYNE2 Polyclonal Antibody detects endogenous levels of protein. |
| Gene Name | SYNE2 KIAA1011 NUA |
| Protein Name | Nesprin-2 (Nuclear envelope spectrin repeat protein 2) (Nucleus and actin connecting element protein) (Protein NUANCE) (Synaptic nuclear envelope protein 2) (Syne-2) |
| Other Name | |
| Species | Human |
| Gene ID | 23224 |
| Uniprot | Q8WXH0, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q6ZWQ0 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus outer membrane; Single-pass type IV membrane protein; Cytoplasmic side . Sarcoplasmic reticulum membrane; Single-pass type IV membrane protein . Cell membrane; Single-pass membrane protein . Cytoplasm, cytoskeleton. Mitochondrion. Nucleus, nucleoplasm. Cytoplasm, myofibril, sarcomere, Z line . Different isoform patterns are found in the different compartments of the cell. The isoforms having the C-terminal transmembrane span can be found in several organellar membranes like the nuclear envelope, the sarcoplasmic reticulum of myoblasts, or the lamellipodia and focal adhesions at the cell membrane. The largest part of the outer nuclear membrane-associated protein is cytoplasmic, while its C-terminal part is associated with the nuclear envelope, most probably the outer nuclear membrane. Remains associated with the nuclear envelope during its breakdown in mitotic cells. Shorter soluble isoforms can be found in the cytoplasm and within the nucleus.; [Isoform 8]: Cell junction, focal adhesion . In U2OS cells. . |
| Tissue Expression | Widely expressed, with higher level in kidney, adult and fetal liver, stomach and placenta. Weakly expressed in skeletal muscle and brain. Isoform 5 is highly expressed in pancreas, skeletal muscle and heart. |
| Signaling_pathway | |
| Research Areas | |
| Function | Domain:The Klarsicht domain mediates the nuclear envelope targeting.,Function:Involved in the maintenance of nuclear organization and structural integrity. Probable anchoring protein which theters the nucleus to the cytoskeleton. Connects nuclei to the cytoskeleton by interacting with the nuclear envelope and with F-actin in the cytoplasm.,similarity:Belongs to the nesprin family.,similarity:Contains 1 actin-binding domain.,similarity:Contains 1 KASH domain.,similarity:Contains 13 LRR (leucine-rich) repeats.,similarity:Contains 2 CH (calponin-homology) domains.,similarity:Contains 9 spectrin repeats.,subcellular location:The largest part of the protein is cytoplasmic, while its C-terminal part is associated with the nuclear envelope, most probably the outer nuclear membrane. Remains associated with the nuclear envelope during its breakdown in mitotic cells.,subunit:Interacts with F-actin via its N-terminal domain.,tissue specificity:Widely expressed, with higher level in kidney, adult and fetal liver, stomach and placenta. Weakly expressed in skeletal muscle and brain. Isoform 5 is highly expressed in pancreas, skeletal muscle and heart., |
