SKU: WM0539 Categories: ,

Syntenin-1 Monoclonal Antibody

Price range: $268.00 through $328.00

SKU: WM0539-50
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Product details

Background:
syndecan binding protein(SDCBP) Homo sapiens The protein encoded by this gene was initially identified as a molecule linking syndecan-mediated signaling to the cytoskeleton. The syntenin protein contains tandemly repeated PDZ domains that bind the cytoplasmic, C-terminal domains of a variety of transmembrane proteins. This protein may also affect cytoskeletal-membrane organization, cell adhesion, protein trafficking, and the activation of transcription factors. The protein is primarily localized to membrane-associated adherens junctions and focal adhesions but is also found at the endoplasmic reticulum and nucleus. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

Specifications

Size50μL/100μL
Targetsyntenin
ReactivityHuman, Rat, Dog, Pig
ApplicationsWB
MW(Calculated)32kD
MW(Observed)
Host SpeciesMouse
Isotype
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:1000-1:2000; Not yet tested in other applications.
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationAffinity purification
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone Number8C6
ImmunogenPurified recombinant human Syntenin-1 protein fragments expressed in E.coli.
SpecificitySyntenin-1 Monoclonal Antibody detects endogenous levels of Syntenin-1 protein.
Gene NameSDCBP
Protein NameSyntenin-1
Other NameSDCBP; / MDA9; / SYCL; / Syntenin-1; / Melanoma differentiation-associated protein 9; / MDA-9; / Pro-TGF-alpha cytoplasmic domain-interacting protein 18; / TACIP18; / Scaffold protein Pbp1; / Syndecan-binding protein 1
SpeciesHuman
Gene ID6386
UniprotO00560,
Species.1Mouse
Gene ID.1
Uniprot.1O08992,
Species.2Rat
Gene ID.283841
Uniprot.2Q9JI92
Cellular LocalizationCell junction, focal adhesion . Cell junction, adherens junction . Cell membrane; Peripheral membrane protein . Endoplasmic reticulum membrane; Peripheral membrane protein . Nucleus . Melanosome . Cytoplasm, cytosol . Cytoplasm, cytoskeleton . Secreted, extracellular exosome . Membrane raft . Mainly membrane-associated. Localized to adherens junctions, focal adhesions and endoplasmic reticulum. Colocalized with actin stress fibers. Also found in the nucleus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Associated to the plasma membrane in the presence of FZD7 and phosphatidylinositol 4,5-bisphosphate (PIP2) (PubMed:27386966). .
Tissue ExpressionExpressed in lung cancers, including adenocarcinoma, squamous cell carcinoma and small-cell carcinoma (at protein level) (PubMed:25893292). Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.
Signaling_pathway
Research Areas
FunctionFunction:Seems to function as an adapter protein. In adherens junctions may function to couple syndecans to cytoskeletal proteins or signaling components. Seems to couple transcription factor SOX4 to the IL-5 receptor (IL5RA). May also play a role in vesicular trafficking. Seems to be required for the targeting of TGFA to the cell surface in the early secretory pathway.,induction:By gamma interferon in melanoma cells.,PTM:Phosphorylated on tyrosine residues.,similarity:Contains 2 PDZ (DHR) domains.,subcellular location:Mainly membrane-associated. Localized to adherens junctions, focal adhesions and endoplasmic reticulum. Colocalized with actin stress fibers. Also found in the nucleus. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Monomer and homodimer (By similarity). Interacts with SDC1, SDC2, SDC3, SDC4, NRXN2, EPHA7, EPHB1, NF2 isoform 1, TGFA and IL5RA. Interacts with neurofascin, SDCBP2 and PTPRJ.,tissue specificity:Widely expressed. Expressed in fetal kidney, liver, lung and brain. In adult highest expression in heart and placenta.,
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