Name: U-Blot® TAK1 Rabbit mAb
SKU: WR4218
Price: 268 USD
Availability: InStock

Product details

Background:The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	Tak1
Reactivity	Human, Mouse, Rat
Applications	WB, IF, ELISA, IHC
MW(Calculated)	67kD
MW(Observed)	67kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IHC 1:100-1:400 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0396R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	MAP3K7
Protein Name	Mitogen-activated protein kinase kinase kinase 7
Other Name	MAP3K7; / TAK1; / Mitogen-activated protein kinase kinase kinase 7; / Transforming growth factor-beta-activated kinase 1; / TGF-beta-activated kinase 1
Species	Human
Gene ID	6885
Uniprot	O43318,
Species.1	Mouse
Gene ID.1	26409
Uniprot.1	Q62073,
Species.2	Rat
Gene ID.2	313121
Uniprot.2	P0C8E4
Cellular Localization	Cytoplasm, Membrane
Tissue Expression	Isoform 1A is the most abundant in ovary, skeletal muscle, spleen and blood mononuclear cells. Isoform 1B is highly expressed in brain, kidney and small intestine. Isoform 1C is the major form in prostate. Isoform 1D is the less abundant form.
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Cellular community - eukaryotes >> Adherens junction;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation;Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> RIG-I-like receptor signaling pathway;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> IL-17 signaling pathway;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway
Research Areas	>>MAPK signaling pathway; / >>NF-kappa B signaling pathway; / >>Autophagy - animal; / >>AMPK signaling pathway; / >>Wnt signaling pathway; / >>Osteoclast differentiation; / >>Adherens junction; / >>Neutrophil extracellular trap formation; / >>Toll-like receptor signaling pathway; / >>NOD-like receptor signaling pathway; / >>RIG-I-like receptor signaling pathway; / >>IL-17 signaling pathway; / >>T cell receptor signaling pathway; / >>TNF signaling pathway; / >>Alcoholic liver disease; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection; / >>Yersinia infection; / >>Leishmaniasis; / >>Toxoplasmosis; / >>Hepatitis B; / >>Measles; / >>Herpes simplex virus 1 infection; / >>Epstein-Barr virus infection; / >>Human immunodeficiency virus 1 infection; / >>Coronavirus disease - COVID-19; / >>Lipid and atherosclerosis; / >>Fluid shear stress and atherosclerosis
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,Function:Component of a protein kinase signal transduction cascade. Mediator of TGF-beta signal transduction. Stimulates NF-kappa-B activation and the p38 MAPK pathway.,PTM:Association with MAP3K7IP1 promotes autophosphorylation and subsequent activation. Dephosphorylation at Thr-187 by PP2A and PPP6C leads to inactivation.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts with MAP3K7IP1 and MAP3K7IP2. Interacts with PPM1L. Interaction with PP2A and PPP6C leads to its' repressed activity.,