Name: U-Blot® TCF7 Rabbit mAb
SKU: WR4444
Price: 268 USD
Availability: InStock

U-Blot® TCF7 Rabbit mAb

U-14 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

WR4444 U-14 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4444-50

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Product details

Background:alternative products:2 series of isoforms, L and S, are produced by use of alternative promoter usage. Additional isoforms seem to exist,function:Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5′-WWCAAAG-3′) found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.,induction:By TCF7L2 and CTNNB1.,sequence caution:Wrong choice of frame.,similarity:Belongs to the TCF/LEF family.,similarity:Contains 1 HMG box DNA-binding domain.,subunit:Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with AES, TLE1, TLE2, TLE3 and TLE4.,tissue specificity:Predominantly in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.,

Specifications

Size	50μL/100μL
Target	TCF7
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	42kD
MW(Observed)	60kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:1000-1:4000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0667R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	TCF7
Protein Name	Transcription factor 7
Other Name	TCF7; / TCF1; / Transcription factor 7; / TCF-7; / T-cell-specific transcription factor 1; / T-cell factor 1; / TCF-1
Species	Human
Gene ID	995
Uniprot	P36402,
Species.1	Mouse
Gene ID.1	21414
Uniprot.1	Q00417
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Nucleus
Tissue Expression	Colon carcinoma,Epithelium,Skin,Testis,
Signaling_pathway	Cellular Processes >> Cell growth and death >> Cell cycle;Human Diseases >> Cancer: overview >> MicroRNAs in cancer
Research Areas	>>Cell cycle; / >>Oocyte meiosis; / >>Progesterone-mediated oocyte maturation; / >>Human immunodeficiency virus 1 infection; / >>MicroRNAs in cancer
Function	Alternative products:2 series of isoforms, L and S, are produced by use of alternative promoter usage. Additional isoforms seem to exist,Function:Transcriptional activator involved in T-cell lymphocyte differentiation. Necessary for the survival of CD4(+) CD8(+) immature thymocytes. Isoforms lacking the N-terminal CTNNB1 binding domain cannot fulfill this role. Binds to the T-lymphocyte-specific enhancer element (5'-WWCAAAG-3') found in the promoter of the CD3E gene. May also act as feedback transcriptional repressor of CTNNB1 and TCF7L2 target genes. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by TCF7 and CTNNB1.,induction:By TCF7L2 and CTNNB1.,sequence Caution:Wrong choice of frame.,similarity:Belongs to the TCF/LEF family.,similarity:Contains 1 HMG box DNA-binding domain.,subunit:Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with AES, TLE1, TLE2, TLE3 and TLE4.,tissue specificity:Predominantly in T-cells. Also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium.,