TFE3 [PT2169] mouse mAb
Ucallm_WM1276-50
Price range: $268.00 through $328.00
Product details
Background:
transcription factor binding to IGHM enhancer 3(TFE3) Homo sapiens This gene encodes a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. This gene may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013],
Specifications
| Size | 50μL/100μL |
| Target | TFE3 |
| Reactivity | Human, Mouse, Bovine |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 62kD |
| MW(Observed) | 62kD |
| Host Species | Mouse |
| Isotype | IgG1,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Monoclonal |
| Clone Number | RMM2169 |
| Immunogen | Synthesized peptide derived from human TFE3 AA range: 100-200 |
| Specificity | This antibody detects endogenous levels of TFE3 protein. |
| Gene Name | TFE3 BHLHE33 |
| Protein Name | Transcription factor E3 (Class E basic helix-loop-helix protein 33) (bHLHe33) |
| Other Name | TFE3; / BHLHE33; / Transcription factor E3; / Class E basic helix-loop-helix protein 33; / bHLHe33 |
| Species | Human |
| Gene ID | 7030 |
| Uniprot | P19532, |
| Species.1 | Mouse |
| Gene ID.1 | 209446 |
| Uniprot.1 | Q64092 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasmic, Nuclear |
| Tissue Expression | Ubiquitous in fetal and adult tissues. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Mitophagy - animal;Human Diseases >> Cancer: overview >> Transcriptional misregulation in cancer;Human Diseases >> Cancer: specific types >> Renal cell carcinoma |
| Research Areas | |
| Function | Transcription factor that acts as a master regulator of lysosomal biogenesis and immune response . Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF (By similarity). Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFE3 phosphorylation by MTOR promotes its cytosolic retention and subsequent inactivation . Upon starvation or lysosomal stress, inhibition of MTOR induces TFE3 dephosphorylation, resulting in nuclear localization and transcription factor activity . In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity (By similarity). Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer . It also binds very well to a USF/MLTF site . May regulate lysosomal positioning in response to nutrient deprivation by promoting the expression of PIP4P1 . Acts as a positive regulator of browning of adipose tissue by promoting expression of target genes; mTOR-dependent phosphorylation promotes cytoplasmic retention of TFE3 and inhibits browning of adipose tissue (By similarity). Maintains the pluripotent state of embryonic stem cells by promoting the expression of genes such as ESRRB; mTOR-dependent nuclear exclusion promotes exit from pluripotency (By similarity). Required to maintain the naive pluripotent state of hematopoietic stem cell; mTOR-dependent cytoplasmic retention of TFE3 promotes the exit of hematopoietic stem cell from pluripotency . |
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