Price range: $268.00 through $328.00
Background:
This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Feb 2010],
| Size | 50μL/100μL |
| Target | THC2 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 97kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MASTL. AA range:821-870 |
| Specificity | THC2 Polyclonal Antibody detects endogenous levels of THC2 protein. |
| Gene Name | MASTL |
| Protein Name | Serine/threonine-protein kinase greatwall |
| Other Name | MASTL; / GW; / GWL; / THC2; / Serine/threonine-protein kinase greatwall; / GW; / GWL; / hGWL; / Microtubule-associated serine/threonine-protein kinase-like; / MAST-L |
| Species | Human |
| Gene ID | 84930 |
| Uniprot | Q96GX5, |
| Species.1 | Mouse |
| Gene ID.1 | 67121 |
| Uniprot.1 | Q8C0P0 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nucleus . Cleavage furrow . During interphase is mainly nuclear, upon nuclear envelope breakdown localizes at the cytoplasm and during mitosis at the centrosomes. Upon mitotic exit moves to the cleavage furrow. . |
| Tissue Expression | Epithelium,Placenta, |
| Signaling_pathway | |
| Research Areas | |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Disease:Defects in MASTL are the cause of thrombocytopenia type 2 (THC2) [MIM:188000]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting.,Function:Putative serine/threonine kinase which may be involved in megakaryocyte differentiation.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain., |
