Price range: $268.00 through $328.00
Background:
The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011],
| Size | 50μL/100μL |
| Target | Thioredoxin |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 12kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC: 100-300; WB 1:500-2000; ELISA 1:10000-20000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human Thioredoxin. at AA range: 52-101 |
| Specificity | This antibody detects endogenous levels of Thioredoxin |
| Gene Name | TXN TRDX TRX TRX1 |
| Protein Name | Thioredoxin |
| Other Name | Thioredoxin; / Trx; / ATL-derived factor; / ADF; / Surface-associated sulphydryl protein; / SASP |
| Species | Human |
| Gene ID | 7295 |
| Uniprot | P10599, |
| Species.1 | Mouse |
| Gene ID.1 | 22166 |
| Uniprot.1 | P10639 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Cytoplasm . Secreted . Translocates from the cytoplasm into the nucleus after phorbol 12-myristate 13-acetate induction (PMA) (PubMed:9108029). Predominantly in the cytoplasm in non irradiated cells (PubMed:11118054). Radiation induces translocation of TRX from the cytoplasm to the nucleus (PubMed:11118054). Secreted by a leaderless secretory pathway (PubMed:1332947). . |
| Tissue Expression | Brain,Cajal-Retzius cell,Cerebellum,Cervix,Epithelium,Fetal brain cortex,Hepatocyte,Lens,Ma |
| Signaling_pathway | Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Human Diseases >> Neurodegenerative disease >> Parkinson disease |
| Research Areas | >>NOD-like receptor signaling pathway; / >>Parkinson disease; / >>Salmonella infection; / >>Fluid shear stress and atherosclerosis |
| Function | Function:ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).,Function:Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity.,PTM:In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins.,similarity:Belongs to the thioredoxin family.,similarity:Contains 1 thioredoxin domain.,subunit:Homodimer; disulfide-linked. Interacts with TXNIP through the redox-active site. Interacts with MAP3K5 and CASP3., |
