SKU: WR4751 Categories: ,

U-Blot® TIAM2 Rabbit mAb

Price range: $268.00 through $328.00

SKU: WR4751-50
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Product details

Background:This gene encodes a guanine nucleotide exchange factor. A highly similar mouse protein specifically activates ras-related C3 botulinum substrate 1, converting this Rho-like guanosine triphosphatase (GTPase) from a guanosine diphosphate-bound inactive state to a guanosine triphosphate-bound active state. The encoded protein may play a role in neural cell development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Specifications

Size50μL/100μL
TargetTIAM2
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)187kD
MW(Observed)187kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM1111R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NameTIAM2,KIAA2016,STEF
Protein NameRho guanine nucleotide exchange factor TIAM2 (SIF and TIAM1-like exchange factor) (T-lymphoma invasion and metastasis-inducing protein 2) (TIAM-2)
Other NameKIAA2016 STEF
SpeciesHuman
Gene ID26230
UniprotQ8IVF5,
Species.1Mouse
Gene ID.124001
Uniprot.1Q6ZPF3
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationSUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q6ZPF3}. Cell projection, lamellipodium {ECO:0000250|UniProtKB:Q6ZPF3}. Cell projection, filopodium {ECO:0000250|UniProtKB:Q6ZPF3}. Cell projection, growth cone {ECO:0000250|UniProtKB:Q6ZPF3}. Cell projection, neuron projection {ECO:0000250|UniProtKB:Q6ZPF3}. Perikaryon {ECO:0000250|UniProtKB:Q6ZPF3}.
Tissue ExpressionTISSUE SPECIFICITY: Expressed in the occipital, frontal and temporal lobes, cerebellum, putamen and testis. {ECO:0000269|PubMed:10512681}.
Signaling_pathway
Research Areas
FunctionFUNCTION: Modulates the activity of RHO-like proteins and connects extracellular signals to cytoskeletal activities. Acts as a GDP-dissociation stimulator protein that stimulates the GDP-GTP exchange activity of RHO-like GTPases and activates them. Mediates extracellular laminin signals to activate Rac1, contributing to neurite growth. Involved in lamellipodial formation and advancement of the growth cone of embryonic hippocampal neurons. Promotes migration of neurons in the cerebral cortex. When overexpressed, induces membrane ruffling accompanied by the accumulation of actin filaments along the altered plasma membrane (By similarity). Activates specifically RAC1, but not CDC42 and RHOA. {ECO:0000250, ECO:0000269|PubMed:10512681}.
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