Price range: $268.00 through $328.00
Background:
This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012],
| Size | 50μL/100μL |
| Target | TOP1M |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 66kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-2000; ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% glycerol,0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from part region of human protein |
| Specificity | TOP1M Polyclonal Antibody detects endogenous levels of protein. |
| Gene Name | TOP1MT |
| Protein Name | DNA topoisomerase I, mitochondrial (TOP1mt) (EC 5.99.1.2) |
| Other Name | |
| Species | Human |
| Gene ID | 116447 |
| Uniprot | Q969P6, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q8R4U6, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q6IM78 |
| Cellular Localization | Mitochondrion . |
| Tissue Expression | Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver. |
| Signaling_pathway | |
| Research Areas | |
| Function | Catalytic activity:ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.,cofactor:Divalent metal ions (calcium or magnesium).,Function:Relieves DNA strain that arise during duplication of mitochondrial DNA.,miscellaneous:When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.,similarity:Belongs to the eukaryotic type I topoisomerase family.,tissue specificity:Ubiquitous; highest in skeletal muscle, heart, brain and fetal liver., |
