Name: U-Blot® TRAF2 Rabbit mAb
SKU: WR4671
Price: 268 USD
Availability: InStock

Product details

Background:TNF receptor associated factor 2(TRAF2) Homo sapiens The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this pro

Specifications

Target	TRAF2
Reactivity	Human, Mouse, Rat, Bovine
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	56kD
MW(Observed)	56kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	PT0965R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Sequence
Specificity	Endogenous
Gene Name	TRAF2
Protein Name	TNF receptor-associated factor 2
Other Name	TRAF2; / TRAP3; / TNF receptor-associated factor 2; / E3 ubiquitin-protein ligase TRAF2; / Tumor necrosis factor type 2 receptor-associated protein 3
Species	Human
Gene ID-1	7186
Uniprot	Q12933,
Species.1	Mouse
Gene ID-2	22030
Uniprot.1	P39429
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	TNF receptor associated factor 2(TRAF2) Homo sapiens The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this pro
Cellular Localization	Cytoplasm .
Tissue Expression	Brain,Cerebellum,Colon,Fetal brain,Human endometrium,Kidney,Leukocyte,Spleen
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Mitophagy - animal;Cellular Processes >> Cell growth and death >> Apoptosis;Cellular Processes >> Cell growth and death >> Necroptosis;Organismal Systems >> Immune system >> NOD-like receptor signaling pathway;Organismal Systems >> Immune system >> RIG-I-like receptor signaling pathway;Organismal Systems >> Immune system >> IL-17 signaling pathway;Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Cancer: overview >> Pathways in cancer;Human Diseases >> Cancer: specific types >> Small cell lung cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Spinocerebellar ataxia;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway;Environmental Information Processing >> Signal transduction >> TNF signaling pathway;Environmental Information Processing >> Signal transduction >> Sphingolipid signaling pathway
Research Areas	>>MAPK signaling pathway; / >>NF-kappa B signaling pathway; / >>Sphingolipid signaling pathway; / >>Protein processing in endoplasmic reticulum; / >>Apoptosis; / >>Necroptosis; / >>Osteoclast differentiation; / >>NOD-like receptor signaling pathway; / >>RIG-I-like receptor signaling pathway; / >>IL-17 signaling pathway; / >>TNF signaling pathway; / >>Adipocytokine signaling pathway; / >>Non-alcoholic fatty liver disease; / >>Alzheimer disease; / >>Amyotrophic lateral sclerosis; / >>Huntington disease; / >>Spinocerebellar ataxia; / >>Pathways of neurodegeneration - multiple diseases; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection; / >>Yersinia infection; / >>Hepatitis C; / >>Human cytomegalovirus infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Herpes simplex virus 1 infection; / >>Epstein-Barr virus infection; / >>Human immunodeficiency virus 1 infection; / >>Pathways in cancer; / >>Viral carcinogenesis; / >>Small cell lung cancer; / >>Lipid and atherosclerosis
Function	Domain:The coiled coil domain mediates homo- and hetero-oligomerization.,Domain:The MATH/TRAF domain binds to receptor cytoplasmic domains.,Function:Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Association to the receptor is also mediated by the interaction with TRADD. Mediates activation of NF-kappa-B and JNK and is involved in apoptosis. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. Seems to be involved in IL-15 signaling.,PTM:Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation. Not ubiquitinated by SIAH1.,similarity:Contains 1 MATH domain.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 2 TRAF-type zinc fingers.,subunit:Homotrimer (Probable). Heteromer with TRAF1. Binds to TNFRSF1B/TNFR2, TNFRSF4 and TNFRSF5/CD40. Associates with CD27, TNFRSF8/CD30, TNFRSF9/CD137, TNFRSF11A/RANK, TNFRSF13B/TACI. TNFRSF14, TNFRSF16/NGFR, TNFRSF17/BCMA, TNFRSF18/AITR, TNFRSF19/TROY, TNFRSF19L/RELT, XEDAR, EDAR, Epstein-Barr virus BNFL1/LMP-1, IL15RA, TANK/ITRAF, RIPK2, TNIK, MAP3K14, MAP3K5, MAP3K1, MAP4K2, CDK9, CSK, and TRAF-interacting protein TRAIP and TRAF and TNF receptor associated protein TTRAP. Interacts with TNFAIP3 and TRPC4AP. Interacts with PEG3 (By similarity). Binds to TRADD. Interacts with BIRC2 and BIRC3 N-terminus. Interacts with CYLD and TBK1. Interacts with MAVS/IPS1. Interacts with CASP8AP2 and USP48. Interacts with DAB2IP. Interacts wtih NFATC2IP and with HIVEP3.,
RRID	AB_3712217