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Home/Reagents/Antibody/Primary antibody/Monoclonal Antibody/Mouse mAb/TRIM5α Monoclonal Antibody
SKU: WM0450 Categories: ,

TRIM5α Monoclonal Antibody

Ucallm_WM0450-50

Price range: $268.00 through $328.00

SKU: WM0450-50
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Product details

Background:
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009],

Specifications

Size50μL/100μL
TargetTRIM5α
ReactivityHuman
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)56kD
MW(Observed)
Host SpeciesMouse
Isotype
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:500-1:2000; IHC 1:200-1:1000; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationAffinity purification
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone Number5A6
ImmunogenPurified recombinant fragment of human TRIM5α expressed in E. Coli.
SpecificityTRIM5α Monoclonal Antibody detects endogenous levels of TRIM5α protein.
Gene NameTRIM5
Protein NameTripartite motif-containing protein 5
Other NameTRIM5; / RNF88; / Tripartite motif-containing protein 5; / RING finger protein 88
SpeciesHuman
Gene ID85363
UniprotQ9C035
Species.1
Gene ID.1
Uniprot.1
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationCytoplasm . Nucleus . Predominantly localizes in cytoplasmic bodies (PubMed:12878161, PubMed:20357094). Localization may be influenced by the coexpression of other TRIM proteins, hence partial nuclear localization is observed in the presence of TRIM22 or TRIM27 (By similarity). In cytoplasmic bodies, colocalizes with proteasomal subunits and SQSTM1 (By similarity). .
Tissue ExpressionBrain,Placenta,Rhabdomyosarcoma,
Signaling_pathway
Research Areas>>Viral life cycle - HIV-1; / >>Human immunodeficiency virus 1 infection
FunctionDomain:The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.,Function:Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity.,pathway:Protein modification; protein ubiquitination.,PTM:Ubiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro).,similarity:Belongs to the TRIM/RBCC family.,similarity:Contains 1 B box-type zinc finger.,similarity:Contains 1 B30.2/SPRY domain.,similarity:Contains 1 RING-type zinc finger.,subcellular location:Cytoplasmic bodies.,subunit:Isoform Alpha forms homotrimers, and may interact with retroviral CA protein. Isoform Delta interacts with BTBD1 and BTBD2.,
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