Specifications
| Size | 50μL/100μL |
| Target | 14-3-3 β |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 28kD |
| MW(Observed) | 28kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1822R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | YWHAB |
| Protein Name | 14-3-3 protein beta/alpha |
| Other Name | YWHAB; / 14-3-3 protein beta/alpha; / Protein 1054; / Protein kinase C inhibitor protein 1; / KCIP-1 |
| Species | Human |
| Gene ID | 7529 |
| Uniprot | P31946, |
| Species.1 | Mouse |
| Gene ID.1 | 54401 |
| Uniprot.1 | Q9CQV8, |
| Species.2 | Rat |
| Gene ID.2 | 56011 |
| Uniprot.2 | P35213 |
| Cellular Localization | Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.; Vacuole membrane . (Microbial infection) Upon infection with Chlamydia trachomatis, this protein is associated with the pathogen-containing vacuole membrane where it colocalizes with IncG. . |
| Tissue Expression | Brain,Colon carcinoma,Kerat |
| Signaling_pathway | Cellular Processes >> Cell growth and death >> Cell cycle;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway |
| Research Areas | >>Cell cycle; / >>Oocyte meiosis; / >>PI3K-Akt signaling pathway; / >>Hippo signaling pathway; / >>Hepatitis C; / >>Hepatitis B; / >>Viral carcinogenesis |
| Function | Function:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.,PTM:Isoform Short contains a N-acetylmethionine at position 1.,PTM:The alpha, brain-specific form differs from the beta form in being phosphorylated.,similarity:Belongs to the 14-3-3 family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Homodimer. Interacts with SSH1 and TORC2/CRTC2. Interacts with ABL1; the interaction results in cytoplasmic location of ABL1 and inhition of cABL-mediated apoptosis. Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues., |
