U-Blot® AR-β2 Rabbit mAb
Ucallm_WR5277-50
Price range: $268.00 through $328.00
Product details
Background:This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Adrenergic Receptor β2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 46kD |
| MW(Observed) | 95kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:1000-1:20000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1522R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | ADRB2 |
| Protein Name | Beta-2 adrenergic receptor |
| Other Name | ADRB2; / ADRB2R; / B2AR; / Beta-2 adrenergic receptor; / Beta-2 adrenoreceptor; / Beta-2 adrenoceptor |
| Species | Human |
| Gene ID | 154 |
| Uniprot | P07550, |
| Species.1 | Mouse |
| Gene ID.1 | 11555 |
| Uniprot.1 | P18762, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P10608 |
| Cellular Localization | Cell membrane; Multi-pass membrane protein . Early endosome . Golgi apparatus . Colocalizes with VHL at the cell membrane (PubMed:19584355). Activated receptors are internalized into endosomes prior to their degradation in lysosomes (PubMed:20559325). Activated receptors are also detected within the Golgi apparatus (PubMed:27481942). . |
| Tissue Expression | Blood,Brain,Fetal brain,Heart,Leukocyte,Prostate,Thyroid, |
| Signaling_pathway | Organismal Systems >> Circulatory system >> Adrenergic signaling in cardiomyocytes;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> cAMP signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway |
| Research Areas | >>Calcium signaling pathway; / >>cGMP-PKG signaling pathway; / >>cAMP signaling pathway; / >>Neuroactive ligand-receptor interaction; / >>Adrenergic signaling in cardiomyocytes; / >>Regulation of lipolysis in adipocytes; / >>Renin secretion; / >>Salivary secretion; / >>Chemical carcinogenesis - receptor activation |
| Function | Disease:Polymorphic forms of ADRB2 could impart some form of nocturnal asthma.,Function:Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.,PTM:Palmitoylated; may reduce accessibility of Ser-345 and Ser-346 by anchoring Cys-341 to the plasma membrane. Agonist stimulation promotes depalmitoylation and further allows Ser-345 and Ser-346 phosphorylation.,PTM:Phosphorylated by PKA and BARK upon agonist stimulation, which mediates homologous desensitization of the receptor. PKA-mediated phosphorylation seems to facilitate phosphorylation by BARK. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Phosphorylation of Tyr-141 is induced by insulin and leads to supersensitization of the receptor.,similarity:Belongs to the G-protein coupled receptor 1 family.,subunit:Binds SLC9A3R1 and GPRASP1., |
Please login to post questions
Related products
