Specifications
| Size | 50μL/100μL |
| Target | BCKDK |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 46kD |
| MW(Observed) | 46kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1812R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | BCKDK |
| Protein Name | [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase mitochondrial |
| Other Name | BCKDK; / [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase; / mitochondrial; / Branched-chain alpha-ketoacid dehydrogenase kinase; / BCKD-kinase; / BCKDHKIN |
| Species | Human |
| Gene ID | 10295 |
| Uniprot | O14874, |
| Species.1 | Mouse |
| Gene ID.1 | 12041 |
| Uniprot.1 | O55028, |
| Species.2 | Rat |
| Gene ID.2 | 29603 |
| Uniprot.2 | Q00972 |
| Cellular Localization | Mitochondrion matrix. Mitochondrion . |
| Tissue Expression | Ubiquitous. |
| Signaling_pathway | |
| Research Areas | |
| Function | Catalytic activity:ATP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] = ADP + [3-methyl-2-oxobutanoate dehydrogenase (acetyl-transferring)] phosphate.,Function:Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.,PTM:Autophosphorylated.,similarity:Belongs to the PDK/BCKDK protein kinase family.,similarity:Contains 1 histidine kinase domain.,subunit:Monomer.,tissue specificity:Ubiquitous., |
