Name: U-Blot® CaMKK2 Rabbit mAb
SKU: WR5887
Price: 268 USD
Availability: InStock

Product details

Background：
The product of this gene belongs to the Serine/Threonine protein kinase family , and to the Ca (2+) /calmodulin-dependent protein kinase subfamily. The major isoform of this gene plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Protein products of this gene also phosphorylate AMP-activated protein kinase (AMPK) . This gene has its strongest expression in the brain and influences signalling cascades involved with learning and memory , neuronal differentiation and migration , neurite outgrowth , and synapse formation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. The identified isoforms differ in their ability to undergo autophosphorylation and to phosphorylate downstream kinases. [provided by RefSeq , Jul 2012] ,

Specifications

Size	50μL/100μL
Target	CaMKK2
Reactivity	Human, Mouse, Rat
Applications	WB, IF, ELISA
MW(Calculated)	65kD
MW(Observed)	60-70kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000-1:20000;
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1598R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	CAMKK2 CAMKKB KIAA0787
Protein Name	Calcium/calmodulin-dependent protein kinase kinase 2
Other Name	CAMKK2; / CAMKKB; / KIAA0787; / Calcium/calmodulin-dependent protein kinase kinase 2; / CaM-KK 2; / CaM-kinase kinase 2; / CaMKK 2; / Calcium/calmodulin-dependent protein kinase kinase beta; / CaM-KK beta; / CaM-kinase kinase beta; / CaMKK beta
Species	Human
Gene ID	10645
Uniprot	Q96RR4,
Species.1	Mouse
Gene ID.1	207565
Uniprot.1	Q8C078,
Species.2	Rat
Gene ID.2	83506
Uniprot.2	O88831
Cellular Localization	Nucleus . Cytoplasm . Cell projection, neuron projection . Predominantly nuclear in unstimulated cells, relocalizes into cytoplasm and neurites after forskolin induction. .
Tissue Expression	Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Autophagy - animal;Organismal Systems >> Endocrine system >> Adipocytokine signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Aging >> Longevity regulating pathway;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway
Research Areas	>>Autophagy - animal; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Adipocytokine signaling pathway; / >>Oxytocin signaling pathway; / >>Alcoholic liver disease; / >>Alcoholism
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Domain:The autoinhibitory domain overlaps with the calmodulin binding region and may be involved in intrasteric autoinhibition.,Domain:The RP domain (arginine/proline-rich) is involved in the recognition of CAMKI and CAMK4 as substrates.,enzyme regulation:Activated by Ca(2+)/calmodulin. Binding of calmodulin may releave intrasteric autoinhibition. Autophosphorylation does not alter activity or regulation by Ca(2+)/calmodulin. In part, activity is independent on Ca(2+)/calmodulin.,Function:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Isoform 1, isoform 2 and isoform 3 phosphorylate CAMK1 and CAMK4. Isoform 3 phosphorylates CAMK1D. Isoform 4, isoform 5 and isoform 6 lacking part of the calmodulin-binding domain are inactive. Seems to be involved in hippocampal activation of CREB1.,PTM:Autophosphorylated.,sequence Caution:Intron retention.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with calmodulin.,tissue specificity:Ubiquitously expressed with higher levels in the brain. Intermediate levels are detected in spleen, prostate, thyroid and leukocytes. The lowest level is in lung.,