U-Blot® Claudin 18.2 Rabbit mAb
Ucallm_WR5214-50
Price range: $268.00 through $328.00
Product details
Background:This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2010],
Specifications
| Size | 50μL/100μL |
| Target | Claudin 18.2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 28kD |
| MW(Observed) | 28kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:500-1:2000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1448R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | CLDN18 UNQ778/PRO1572 |
| Protein Name | Claudin-18 |
| Other Name | Claudin-18 |
| Species | Human |
| Gene ID | 51208 |
| Uniprot | P56856-2, |
| Species.1 | Mouse |
| Gene ID.1 | 56492 |
| Uniprot.1 | P56857 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cell junction, tight junction . Cell membrane; Multi-pass membrane protein . Localizes to tight junctions in epithelial cells. . |
| Tissue Expression | Isoform A1: Expression is restricted to the lung (PubMed:19047087). Isoform A2: Expression is restricted to the stomach mucosa where it is predominantly observed in the epithelial cells of the pit region and the base of the gastric glands including exocrine and endocrine cells (at protein level) (PubMed:19047087). |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules |
| Research Areas | >>Cell adhesion molecules; / >>Tight junction; / >>Leukocyte transendothelial migration; / >>Pathogenic Escherichia coli infection; / >>Hepatitis C |
| Function | Function:Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.,similarity:Belongs to the claudin family.,tissue specificity:Concentrated at the cell-cell borders of epithelial cells., |
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