SKU: WR5301 Categories: ,

U-Blot® Claudin-2 Rabbit mAb

Price range: $268.00 through $328.00

SKU: WR5301-50
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Product details

Background:This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.[provided by RefSeq, Jan 2010],

Specifications

Size50μL/100μL
TargetClaudin-2
ReactivityHuman, Mouse
ApplicationsWB, IHC, IF, ELISA
MW(Calculated)25kD
MW(Observed)20kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:200-1:500; WB 1:10000-1:50000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM1483R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NameCLDN2
Protein NameClaudin-2
Other NameCLDN2; / PSEC0059; / SP82; / Claudin-2; / SP82
SpeciesHuman
Gene ID9075
UniprotP57739,
Species.1Mouse
Gene ID.112738
Uniprot.1O88552
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationCell junction, tight junction . Cell membrane; Multi-pass membrane protein .
Tissue ExpressionColon,Colon epithelium,Kidney,Skin,Teratocarcinoma,Thalamus,
Signaling_pathwayCellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules
Research Areas>>Cell adhesion molecules; / >>Tight junction; / >>Leukocyte transendothelial migration; / >>Pathogenic Escherichia coli infection; / >>Hepatitis C
FunctionFunction:Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.,similarity:Belongs to the claudin family.,subunit:Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN1, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3.,
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