Specifications
| Target | -- |
| Reactivity | Human |
| Applications | CUT&Tag; ChIP; ChIP-Seq; TIP-ChIP; WB |
| MW(Calculated) | 120 kDa |
| Host Species | Rabbit |
| Isotype | IgG |
| Recommended Dilution Ratio | Applications Validated by Active Motif: ChIP-Seq: 4 µg per ChIP WB: 0.1-1 µg/ml CUT&Tag: 1 µl per 50 µl reaction TIP-ChIP: 2 µg Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts. |
| Formulation | Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 40% glycerol. Sodium azide is highly toxic. |
| Purification | Protein A Chromatography |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
| Concentration | -- |
| Clonality | Recombinant |
| Clone Number | -- |
| Immunogen | This antibody was raised against a peptide within human CTCF. |
| Specificity | -- |
| Gene Name | -- |
| Other Name | CCCTC-Binding Factor, MRD21 |
| Gene ID | -- |
| Cellular Localization | -- |
| Function | -- |
