Specifications
| Size | 50μL/100μL |
| Target | CYP11A1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 60kD |
| MW(Observed) | 55kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1913R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | CYP11A1 |
| Protein Name | Cholesterol side-chain cleavage enzyme mitochondrial |
| Other Name | CYP11A1; / CYP11A; / Cholesterol side-chain cleavage enzyme; / mitochondrial; / CYPXIA1; / Cholesterol desmolase; / Cytochrome P450 11A1; / Cytochrome P450; / scc |
| Species | Human |
| Gene ID | 1583 |
| Uniprot | P05108, |
| Species.1 | Mouse |
| Gene ID.1 | 14205 |
| Uniprot.1 | P97946, |
| Species.2 | Rat |
| Gene ID.2 | 360457 |
| Uniprot.2 | O35251 |
| Cellular Localization | Mitochondrion inner membrane; Peripheral membrane protein . Localizes to the matrix side of the mitochondrion inner membrane. . |
| Tissue Expression | Brain,Choriocarcinoma,Placenta, |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Aldosterone synthesis and secretion |
| Research Areas | >>Steroid hormone biosynthesis; / >>Metabolic pathways; / >>Ovarian steroidogenesis; / >>Aldosterone synthesis and secretion; / >>Cortisol synthesis and secretion; / >>Cushing syndrome |
| Function | Catalytic activity:Cholesterol + reduced adrenal ferredoxin + O(2) = pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin + H(2)O.,cofactor:Heme group.,Disease:Defects in CYP11A1 are a cause of congenital adrenal insufficiency (CAI).,Disease:Defects in CYP11A1 are a cause of congenital lipoid adrenal hyperplasia (CLAH) [MIM:201710]; also called lipoid CAH. CLAH is the most severe form of adrenal hyperplasia. This autosomal recessive and potentially lethal condition includes the onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. CLAH is a rare disease, except in Japan and Korea where it accounts for a significant percentage of cases of congenital adrenal hyperplasia.,Function:Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.,induction:By 8-bromo cyclic AMP.,pathway:Lipid metabolism; C21-steroid hormone metabolism.,similarity:Belongs to the cytochrome P450 family., |
