U-Blot® EFP Rabbit mAb
Ucallm_WR5949-50
Price range: $268.00 through $328.00
Product details
Background：
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains , a RING , a B-box type 1 and a B-box type 2 , and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor , similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen , and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq , Jul 2008] ,
Specifications
| Size | 50μL/100μL |
| Target | EFP |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 71kD |
| MW(Observed) | 71kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1854R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | TRIM25 |
| Protein Name | E3 ubiquitin/ISG15 ligase TRIM25 |
| Other Name | TRIM25; / EFP; / RNF147; / ZNF147; / E3 ubiquitin/ISG15 ligase TRIM25; / Estrogen-responsive finger protein; / RING finger protein 147; / Tripartite motif-containing protein 25; / Ubiquitin/ISG15-conjugating enzyme TRIM25; / Zinc finger protein 147 |
| Species | Human |
| Gene ID | 7706 |
| Uniprot | Q14258, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q61510 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Cytoplasm, Stress granule . Nucleus . |
| Tissue Expression | Expressed in breast tumors (at protein level). Ubiquitous. |
| Signaling_pathway | Organismal Systems >> Immune system >> RIG-I-like receptor signaling pathway;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway |
| Research Areas | >>NF-kappa B signaling pathway; / >>RIG-I-like receptor signaling pathway; / >>Influenza A |
| Function | Function:Mediates estrogen action in various target organs.,similarity:Contains 1 B30.2/SPRY domain.,similarity:Contains 1 RING-type zinc finger.,tissue specificity:Ubiquitous., |
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