U-Blot® Fibrinogen β Rabbit mAb
Ucallm_WR6047-50
Price range: $268.00 through $328.00
Product details
Background：
The protein encoded by this gene is the beta component of fibrinogen , a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury , fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition , various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading , display vasoconstrictor and chemotactic activities , and are mitogens for several cell types. Mutations in this gene lead to several disorders , including afibrinogenemia , dysfibrinogenemia , hypodysfibrinogenemia and thrombotic tendency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq , Jun 2014] ,
Specifications
| Size | 50μL/100μL |
| Target | Fibrinogen β |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 56kD |
| MW(Observed) | 55kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM2044R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | FGB |
| Protein Name | Fibrinogen beta chain |
| Other Name | FGB; / Fibrinogen beta chain |
| Species | Human |
| Gene ID | 2244 |
| Uniprot | P02675, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q8K0E8 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Secreted . |
| Tissue Expression | Detected in blood plasma (at protein level). |
| Signaling_pathway | Organismal Systems >> Immune system >> Complement and coagulation cascades;Organismal Systems >> Immune system >> Platelet activation;Organismal Systems >> Immune system >> Neutrophil extracellular trap formation |
| Research Areas | >>Complement and coagulation cascades; / >>Platelet activation; / >>Neutrophil extracellular trap formation; / >>Coronavirus disease - COVID-19 |
| Function | Disease:Defects in FGB are a cause of congenital afibrinogenemia [MIM:202400]. This rare autosomal recessive disorder is characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen.,Disease:Defects in FGB are a cause of thrombophilia.,Domain:A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure.,Function:Fibrinogen has a double Function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.,online information:Fibrinogen entry,online information:The Singapore human mutation and polymorphism database,PTM:Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.,similarity:Contains 1 fibrinogen C-terminal domain.,subunit:Heterohexamer; disulfide linked. Contains 2 sets of 3 non-identical chains (alpha, beta and gamma). The 2 heterotrimers are in head to head conformation with the N-termini in a small central domain., |
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