U-Blot® HBO1 Rabbit mAb
Ucallm_WR5421-50
Price range: $268.00 through $328.00
Product details
Background:catalytic activity:Acetyl-CoA + histone = CoA + acetylhistone.,domain:The C2HC-type zinc finger is required for interaction with MCM2 and ORC1L.,domain:The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction.,function:Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MYST (SAS/MOZ) family.,similarity:Contains 1 C2HC-type zinc finger.,subunit:Component of the HBO1 complex composed at least of ING4 or ING5, MYTS2/HBO1, EAF6, and one of PHF15, PHF16 and PHF17. Interacts with MCM2 and ORC1L. Interacts with the androgen receptor (AR) in the presence of dihydrotestosterone.,tissue specificity:Ubiquitously expressed, with highest levels in testis.,
Specifications
| Size | 50μL/100μL |
| Target | HBO1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 71kD |
| MW(Observed) | 71kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1670R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | KAT7 |
| Protein Name | Histone acetyltransferase KAT7 |
| Other Name | KAT7; / HBO1; / HBOa; / MYST2; / Histone acetyltransferase KAT7; / Histone acetyltransferase binding to ORC1; / Lysine acetyltransferase 7; / MOZ; / YBF2/SAS3, SAS2 and TIP60 protein 2; / MYST-2 |
| Species | Human |
| Gene ID | 11143 |
| Uniprot | O95251, |
| Species.1 | Mouse |
| Gene ID.1 | 217127 |
| Uniprot.1 | Q5SVQ0, |
| Species.2 | Rat |
| Gene ID.2 | 303470 |
| Uniprot.2 | Q810T5 |
| Cellular Localization | Nucleus . Chromosome . Chromosome, centromere . Cytoplasm, cytosol . Associates with replication origins specifically during the G1 phase of the cell cycle (PubMed:18832067, PubMed:20129055). Localizes to transcription start sites (PubMed:21753189, PubMed:24065767). Localizes to ultraviolet-induced DNA damage sites following phosphorylation by ATR (PubMed:28719581). Localizes to centromeres in G1 phase (PubMed:27270040). . |
| Tissue Expression | Ubiquitously expressed, with highest levels in testis. |
| Signaling_pathway | |
| Research Areas | |
| Function | Catalytic activity:Acetyl-CoA + histone = CoA + acetylhistone.,Domain:The C2HC-type zinc finger is required for interaction with MCM2 and ORC1L.,Domain:The N-terminus is involved in transcriptional repression, while the C-terminus mediates AR-interaction.,Function:Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may regulate DNA replication and act as a coactivator of TP53-dependent transcription. Specifically represses AR-mediated transcription.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the MYST (SAS/MOZ) family.,similarity:Contains 1 C2HC-type zinc finger.,subunit:Component of the HBO1 complex composed at least of ING4 or ING5, MYTS2/HBO1, EAF6, and one of PHF15, PHF16 and PHF17. Interacts with MCM2 and ORC1L. Interacts with the androgen receptor (AR) in the presence of dihydrotestosterone.,tissue specificity:Ubiquitously expressed, with highest levels in testis., |
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