U-Blot® Histone H2A.X Rabbit pAb

Price range: $700.00 through $1,750.00

SKU: WT8629-100
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Product details

Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015],

Specifications

TargetHistone H2A.X
ReactivityHuman
ApplicationChIP,WB,IF,ICC
MW(Calculated)
MW(Observed)15 kDa
Host SpeciesRabbit
IsotypeIgG
ConjugateUnconjugated
Modification
Modification site
Recommended dilution ratioWB 1:500 - 1:2,000 dilution; ICC/IF 1:500 - 1:1,000 dilution
FormulationPurified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide.
Source
PurificationAffinity Purified
Purity
Storage -20°C/2 year. Avoid freeze/thaw cycles.
Concentration
ClonalityPolyclonal
Clone Number
ImmunogenThis Histone H2AX antibody was raised against a peptide derived from the C-terminus of human histone H2AX.
Sequence
Specificity
Gene nameH2AFX
Protein nameHistone H2A.x
Alternative nameH2AFX;H2AX;Histone H2A.x;H2a/x
Organism-1Human
Gene ID‑13014
SwissProt-1P16104
Organism-2Mouse
Gene ID‑215270
SwissProt-2P27661
Organism-3
Gene ID‑3
SwissProt-3
Organism-4
Gene ID‑4
SwissProt-4
Cellular Localization
Unnamed: 38
Unnamed: 39
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