Price range: $888.00 through $999.00
Background：
Histone serine phosphorylation is a reversible modification controlled by kinases and phosphatases. Phosphorylation alters chromatin electrostatic state and recruits downstream functional proteins to regulate apoptosis, DNA damage repair and cell cycle. Each serine site owns distinct biological roles.
| Target | Phospho-Histone H2B (Ser14) |
| Reactivity | Human Vertebrates |
| Application | WB, ICC, ELISA, Luminex |
| MW(Calculated) | 14kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | Rabbit IgG |
| Conjugate | Unconjugated |
| Modification | Phospho |
| Modified site | Ser14 |
| Recommended dilution ratio | WB 0.5 ug/mL - 2 ug/mL; ICC 1 μg/mL - 2 μg/mL; ELISA 0.2 ug/mL - 1 ug/mL; Luminex 0.1 ug/mL – 1 ug/mL. |
| Composition | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Source | |
| Purification process | Protein A affinity purified from an animal origin-free culture supernatant |
| Purity | |
| Storage | -20.0°C |
| Concentration | 1 mg/mL |
| Clonality | Recombinant Monoclonal |
| Clone Number | WR-227 |
| Immunogen | A phospho-peptide corresponding to Phospho-Histone H2B (Ser14). |
| Sequence | |
| Specificity | This antibody reacts to Histone H2B only when phosphorylated at serine 14. No cross reactivity with other phosphorylated histones. |
| Gene name | |
| Protein Name | |
| Alternative name | H2BpS14 |
| Organism-1 | Human |
| Gene ID-1 | 255626;3018;3017;8339;8343;8344;8346;8347 |
| SwissProt-1 | Q96A08;P33778;P62807 |
| Organism-2 | Mouse |
| Gene ID-2 | 319177;319178;319179 |
| SwissProt-2 | |
| Organism-3 | Rat |
| GeneID-3 | 24829 |
| SwissProt-3 | Q00729 |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Cellular Localization |
