U-Blot® Histone H4 (Acetyl Lys20) Rabbit mAb
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Price range: $400.00 through $1,000.00
Product details
Background:Histone H4 assembles with H3 to form H3-H4 tetramer, the core scaffold of nucleosome. Reversible lysine acetylation on H4 N-terminal tail and core domain is regulated by HAT and HDAC. Acetylation eliminates positive charge of histone tails, loosens chromatin and recruits bromodomain co-activators. Different acetylated lysine sites on H4 perform unique functions in gene transcription, DNA replication, DNA damage repair and epigenetic silencing regulation. H4 Lys20 acetylation (H4K20ac) is catalyzed by p300/CBP. Distinct from repressive H4K20me marks, H4K20ac correlates with transcriptional activation at promoters and enhancers. It participates in cell cycle progression and DNA damage signal transduction; HDAC inhibitor treatment significantly elevates global H4K20ac abundance.
Specifications
| Target | Histone H4 |
| Reactivity | Human,Mouse |
| Application | ICC, WB, ChIP |
| MW(Calculated) | |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Modification | Acetyl |
| Modification site | Lys20 |
| Recommended dilution ratio | Dot Blot 1:1000; WB 1:1000; ICC 1:500; ChIP 1:20‑1:50 |
| Formulation | PBS, 40% Glycerol, 0.05% BSA, 0.03% ProClin 300 |
| Source | |
| Purification | Protein A |
| Purity | |
| Storage | 12 months from date of receipt / reconstitution, -20 ℃ as supplied |
| Concentration | 0.5 mg/ml |
| Clonality | Recombinant Monoclonal |
| Clone Number | WR-255 |
| Immunogen | Synthetic Peptide |
| Sequence | |
| Specificity | |
| Gene name | |
| Protein name | Histone H4 |
| Alternative name | H4K20ac |
| Organism-1 | |
| Gene ID‑1 | |
| SwissProt-1 | |
| Organism-2 | |
| Gene ID‑2 | |
| SwissProt-2 | |
| Organism-3 | |
| Gene ID‑3 | |
| SwissProt-3 | |
| Organism-4 | |
| Gene ID‑4 | |
| SwissProt-4 | |
| Cellular Localization | |
| Unnamed: 38 | |
| Unnamed: 39 |
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