Specifications
| Size | 50μL/100μL |
| Target | HPK1 |
| Reactivity | Human |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 97kD |
| MW(Observed) | 95kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1730R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | MAP4K1 |
| Protein Name | Mitogen-activated protein kinase kinase kinase kinase 1 |
| Other Name | MAP4K1; / HPK1; / Mitogen-activated protein kinase kinase kinase kinase 1; / Hematopoietic progenitor kinase; / MAPK/ERK kinase kinase kinase 1; / MEK kinase kinase 1; / MEKKK 1 |
| Species | Human |
| Gene ID | 11184 |
| Uniprot | Q92918, |
| Species.1 | Mouse |
| Gene ID.1 | 26411 |
| Uniprot.1 | P70218 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | intracellular,cytoplasm,membrane, |
| Tissue Expression | Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> MAPK signaling pathway |
| Research Areas | >>MAPK signaling pathway |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,Function:May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.,similarity:Contains 1 CNH domain.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with MAP3K1.,tissue specificity:Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine., |
