Name: U-Blot® HPRT Rabbit mAb
SKU: WR5885
Price: 268 USD
Availability: InStock

U-Blot® HPRT Rabbit mAb

Ucallm_WR5885-50

Price range: $268.00 through $328.00

Size

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50μl

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SKU: WR5885-50

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Product details

Background：
hypoxanthine phosphoribosyltransferase 1 (HPRT1) Homo sapiens The protein encoded by this gene is a transferase , which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq , Jun 2009] ,

Specifications

Size	50μL/100μL
Target	HPRT
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	25kD
MW(Observed)	25kD
Host Species	Rabbit
Isotype	IgG, Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year (Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1513R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	HPRT1
Protein Name	Hypoxanthine-guanine phosphoribosyltransferase
Other Name	HPRT1; / HPRT; / Hypoxanthine-guanine phosphoribosyltransferase; / HGPRT; / HGPRTase
Species	Human
Gene ID	3251
Uniprot	P00492,
Species.1	Mouse
Gene ID.1
Uniprot.1	P00493
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cytoplasm.
Tissue Expression	Brain ,
Signaling_pathway
Research Areas	>>Purine metabolism; / >>Drug metabolism - other enzymes; / >>Metabolic pathways; / >>Nucleotide metabolism
Function	Catalytic activity:GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate. ,Catalytic activity:IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate. ,cofactor:Binds 2 magnesium ions per subunit. One of the ions does not make direct protein contacts. ,Disease:Defects in HPRT1 are the cause of gout [MIM:300323]; also known as HPRT-related gout or Kelley-Seegmiller syndrome. Gout is characterized by partial enzyme activity and hyperuricemia. ,Disease:Defects in HPRT1 are the cause of Lesch-Nyhan syndrome (LNS) [MIM:300322]. LNS is characterized by complete lack of enzymatic activity that results in hyperuricemia , choreoathetosis , mental retardation , and compulsive self-mutilation. ,online information:Hypoxanthine-guanine phosphoribosyltransferase entry ,pathway:Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1. ,similarity:Belongs to the purine/pyrimidine phosphoribosyltransferase family. ,subunit:Homotetramer. ,